EIF4E antibody (C-Term)

Catalog No. ABIN7540529

Supplier Product No.: 200-901-mk9s

This Chicken Polyclonal antibody specifically detects EIF4E in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for EIF4E antibody (C-Term) (ABIN7540529)

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Reactivity: Human

Host: Chicken

Clonality: Polyclonal

Conjugate: This EIF4E antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-EIF4E Antibody

Binding Specificity: C-Term

Supplier Product No.: 200-901-mk9s

Supplier: Rockland

Purpose: EIF4E Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human EIF4E.

Purification: This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen: Anti-EIF4E antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to a near C-Terminal portion of human EIF4E conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: User Optimized

Immunohistochemistry_Dilution: 1:1000

Western_Blot_Dilution: 5 μg/mL

Comment:

Anti-EIF4E Antibody has been tested in Western Blot and IHC.  Expect a band at ~25-28kDa in western blot using appropriate tissues and lysates.  Positive control used: HEK293T, MOLT-4 and Daudi lysates in Western Blot, Human Lung Adenocarcinoma Tissue​ in Immunohistochemistry.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.002 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.0

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for EIF4E

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Alternative Name: EIF4E

Background: Chicken Anti-EIF4E Antibody, Eukaryotic Translation Initiation Factor 4E, MRNA Cap-Binding Protein, EIF-4F 25 KDa Subunit, EIF4EL1, EIF-4E, EIF4F, Eukaryotic Translation Initiation Factor 4E-Like 1, AUTS19, EIF4E1, EIF4E, CBP,EIF4E (Eukaryotic Translation Initiation Factor 4E) recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. It is a component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap. EIF4E is associated with Autism 19 and Pervasive Developmental Disorder. Anti-EIF4E Antibody is useful for researcher interested in cancer research and AKT signaling.

Gene ID: 1977

NCBI Accession: NP_001124150

UniProt: P06730

Pathways: BCR Signaling