SLC1A3 antibody (N-Term)

Catalog No. ABIN7540531

Supplier Product No.: 200-901-ml1s

Product Details anti-SLC1A3 Antibody

Target: SLC1A3 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3))

Binding Specificity: N-Term

Reactivity: Rat

Host: Chicken

Clonality: Polyclonal

Conjugate: This SLC1A3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Supplier Product No.: 200-901-ml1s

Supplier: Rockland

Purpose: Eaat1 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against rat Slc1a3.

Purification: This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen: Anti-Slc1a3 antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to a N-Terminal portion of rat Slc1a3 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000-1:50,000

IF_Microscopy_Dilution: 15 μg/mL

Western_Blot_Dilution: 1:500

Comment:

Anti-Slc1a3 Antibody has been tested in Western Blot and IF.  Expect a band at ~54.4kDa in western blot using appropriate lysates.  Positive control used: PND2-6 rat brain lysate in Western Blot and PND1 rat brain cells in Immunofluorescence.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.002 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.0

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for SLC1A3

Target: SLC1A3 (Solute Carrier Family 1 (Glial High Affinity Glutamate Transporter), Member 3 (SLC1A3))

Alternative Name: Slc1a3 (SLC1A3 Products)

Background: Chicken Anti-Slc1a3 Antibody, Chicken Anti-Eaat1 Antibody, Excitatory amino acid transporter 1, Glial glutamate transporter, Sodium-dependent glutamate/aspartate transporter 1, GLAST, GAST-1, Slc1a3, Eaat1,Slc1a3 (or Eaat1) is widely distributed throughout the brain. It is a potassium and sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate, L-aspartate, and D-aspartate. It functions as a symporter that transports one amino acid molecule together with two or three Na+ ions and one proton, in parallel with the counter-transport of one K+ ion. It mediates Cl(-) flux that is not coupled to amino acid transport, this avoids the accumulation of negative charges due to aspartate and Na(+) symport. Slc1a3 plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate. Slc1a3 can play a role in Episodic Ataxia, Type 6 and Wernicke Encephalopathy. Anti-Slc1a3 Antibody is useful for researchers interested in SIDS, Glucose Metabolism, and Glutamate and Amino Acid Binding.

Gene ID: 29483

NCBI Accession: NP_001276870

UniProt: P24942

Pathways: Sensory Perception of Sound, Synaptic Membrane, Dicarboxylic Acid Transport