EBI3 antibody

Catalog No. ABIN7540534

Supplier Product No.: 209-401-b66

This anti-EBI3 antibody is a Rabbit Polyclonal antibody detecting EBI3 in WB and ELISA. Suitable for Human.

Quick Overview for EBI3 antibody (ABIN7540534)

Target: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EBI3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-EBI3 Antibody

Supplier Product No.: 209-401-b66

Supplier: Rockland

Purpose: IL-35 EBI3 Antibody

Cross-Reactivity (Details): This antibody is specific for human EBI3 protein.

Purification: Anti-EBI-3 Antibody / Anti-IL27 is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen: Anti-EBI-3 Antibody / Anti-IL27 is an IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with mature length recombinant human EBI3 protein produced in E.coli.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Other: User Optimized

Comment:

Anti-EBI-3 Antibody / Anti-IL27 is a purified antibody that has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 23 kDa in size corresponding to the mature human EBI3 protein by western blotting in appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store Anti-EBI-3 Antibody / Anti-IL27 beta at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 6 months

Target Details for EBI3

Target: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Alternative Name: EBI3

Background: Rabbit Anti-EBI3, rabbit anti-EBI-3, Interleukin-27 subunit beta, IL-27 subunit beta, IL-27B, Epstein-Barr virus-induced gene 3 protein, EBV-induced gene 3 protein, EBI-3, EBI3, IL-35, IL35,The cytokine Interleukin 27 (IL-27) is produced in response to inflammation. It is made by activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells. IL-27 consists of a heterodimeric combination of Epstein-Barr virus-induced molecule 3 (EBI3, or IL-27B) non-covalently linked with IL-27 p28 (or IL-27A). It is a regulator of T helper cell development and suppressor of T-cell proliferation. IL-27 has both pro- and anti-inflammatory properties. It can stimulate cytotoxic T cell activity and induce isotype switching in B-cells. It has diverse effects on innate immune cells. It induces monocytes and mast cells to secrete pro-inflammatory cytokines. When infection is present, IL-27 induces naive CD4+ T cells to proliferate and develop Th1 cell responses. As an anti-inflammatory regulator, IL-27 can inhibit Th1 or Th2 responses and restrict the strength and duration of adaptive immune responses. The IL-27 p28 subunit, a 28 kDa glycoprotein belonging to the type I cytokine family, is homologous to IL-12 p35, IL-23 p19, and IL-6. The EBI3 (Epstein-Barr virus-induced molecule 3, or IL-27B) subunit is a 34 kDa glycoprotein containing two fibronectin type III domains, and belongs to the type I cytokine receptor family. It can exist as a homodimer and can also heterodimerize with IL-27 p28 or IL-12 p35 subunit. It is homologous to the p40 subunit of IL-12 and IL-23 and to the extracellular domain of IL-6 R.

Gene ID: 10148

NCBI Accession: NP_005746

UniProt: Q14213