EBI3 antibody

Catalog No. ABIN7540535

Supplier Product No.: dup_210-301-b66_dup

This Mouse Monoclonal antibody specifically detects EBI3 in WB, ELISA and IP. It exhibits reactivity toward Mouse and has been mentioned in 2+ publications.

Quick Overview for EBI3 antibody (ABIN7540535)

Target: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This EBI3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Clone: V1-4C4-22

Product Details anti-EBI3 Antibody

Supplier Product No.: dup_210-301-b66_dup

Supplier: Rockland

Purpose: IL-35 EBI3 Antibody

Cross-Reactivity (Details): This antibody is specific for mouse EBI3 protein.

Purification: Anti-Mouse EBI-3 (MOUSE) Monoclonal Antibody was purified from mouse ascites by Protein A chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen: Anti-EBI-3 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant mouse EBI-3 produced in E.coli followed by hybridoma development.

Isotype: IgG2b

Application Details

Application Notes:

Neutralization_Dilution: 10 μg/mL

Immunoprecipitation_Dilution: 1 μg/mL

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Other: User Optimized

Comment:

Suggested Applications: Cellular Assay
Anti-Mouse EBI-3 antibody has been tested for use in ELISA and western Blot and is suitable for IP and Neutralization. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-EBI3 antibody (ABIN7540535)

Feng, Zhang, Ding, Chen, Wang, Wang, Zou, Sheng, Chen, Pang, Marshall, Shi, Nedergaard, Li, Xiao: "B lymphocytes ameliorate Alzheimer's disease-like neuropathology via interleukin-35." in: Brain, behavior, and immunity, Vol. 108, pp. 16-31, (2023) (PubMed).

Zhang, Li, Zhou, Zhang, Wu, Xu, Shen, An, Shen, Yang, Zhang, Tao: "The Unknown Aspect of BAFF: Inducing IL-35 Production by a CD5+CD1dhiFcγRIIbhi Regulatory B-Cell Subset in Lupus." in: The Journal of investigative dermatology, Vol. 137, Issue 12, pp. 2532-2543, (2017) (PubMed).

Target Details for EBI3

Target: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Alternative Name: Ebi3

Background: Mouse anti-EBI3 antibody, mouse anti-IL-35 antibody, Epstein-Barr virus induced 3 protein, Interleukin-27 subunit beta, IL-27 subunit beta, IL-27B, Epstein-Barr virus-induced gene 3 protein, EBV-induced gene 3 protein, EBI-3, EBI3, IL-35, IL35,The cytokine Interleukin 27 (IL-27) is produced in response to inflammation. It is made by activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells. IL-27 consists of a heterodimeric combination of Epstein-Barr virus-induced molecule 3 (EBI3, or IL-27B) non-covalently linked with IL-27 p28 (or IL-27A). It is a regulator of T helper cell development and suppressor of T-cell proliferation. IL-27 has both pro- and anti-inflammatory properties. It can stimulate cytotoxic T cell activity and induce isotype switching in B-cells. It has diverse effects on innate immune cells. It induces monocytes and mast cells to secrete pro-inflammatory cytokines. When infection is present, IL-27 induces naive CD4+ T cells to proliferate and develop Th1 cell responses. As an anti-inflammatory regulator, IL-27 can inhibit Th1 or Th2 responses and restrict the strength and duration of adaptive immune responses. The IL-27 p28 subunit, a 28 kDa glycoprotein belonging to the type I cytokine family, is homologous to IL-12 p35, IL-23 p19, and IL-6. The EBI3 (Epstein-Barr virus-induced molecule 3, or IL-27B) subunit is a 34 kDa glycoprotein containing two fibronectin type III domains, and belongs to the type I cytokine receptor family. It can exist as a homodimer and can also heterodimerize with IL-27 p28 or IL-12 p35 subunit. It is homologous to the p40 subunit of IL-12 and IL-23 and to the extracellular domain of IL-6 R.

Gene ID: 50498

NCBI Accession: NP_056581

UniProt: O35228