ALDH1L1 antibody (Internal Region)

Catalog No. ABIN7540557

Supplier Product No.: 600-101-hb6

The Goat Polyclonal anti-ALDH1L1 antibody has been validated for WB, IHC, ELISA, IF and FM. It is suitable to detect ALDH1L1 in samples from Mouse. There are 2+ publications available.

Quick Overview for ALDH1L1 antibody (Internal Region) (ABIN7540557)

Target: ALDH1L1 (Aldehyde Dehydrogenase 1 Family, Member L1 (ALDH1L1))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This ALDH1L1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Product Details anti-ALDH1L1 Antibody

Binding Specificity: Internal Region

Supplier Product No.: 600-101-hb6

Supplier: Rockland

Purpose: Aldh1l1 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against mouse Aldh1l1.

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-Aldh1l1 antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of mouse Aldh1l1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000-1:50,000

Immunohistochemistry_Dilution: 1:200

IF_Microscopy_Dilution: 15 μg/mL

Western_Blot_Dilution: 1:1000

Comment:

Anti-Aldh1l1 Antibody has been tested in WB, IHC, and IF.  Expect a band at ~98.7kDa in western blot using appropriate lysates.  Positive control used: NIH3T3 and Ms Liver for WB, Ms Liver and Kidney for IHC, and NIH3T3 for IF.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-ALDH1L1 antibody (ABIN7540557)

Williamson, Fuertes, Dunn, Drew, Jones: "Reactive astrocytes facilitate vascular repair and remodeling after stroke." in: Cell reports, Vol. 35, Issue 4, pp. 109048, (2022) (PubMed).

Hirai, Miwa, Tanaka, Toriumi, Kunii, Shimbo, Sakamoto, Hino, Izumi, Nagaoka, Yabe, Nakamachi, Shioda, Dan, Miyata, Nishito, Suzuki, Miyashita, Tomoda, Hikida, Horiuchi, Itokawa, Arai, Okado: "High-sucrose diets contribute to brain angiopathy with impaired glucose uptake and psychosis-related higher brain dysfunctions in mice." in: Science advances, Vol. 7, Issue 46, pp. eabl6077, (2021) (PubMed).

Target Details for ALDH1L1

Target: ALDH1L1 (Aldehyde Dehydrogenase 1 Family, Member L1 (ALDH1L1))

Alternative Name: Aldh1l1

Background: Goat Anti-Aldh1l1 Antibody, Cytosolic 10-formyltetrahydrofolate dehydrogenase, 10-FTHFDH, FDH, Aldehyde dehydrogenase family 1 member L1, Fthfd,Aldh1l1, also known as Aldehyde Dehydrogenase 1 Family Member L1, belongs to the aldehyde dehydrogenase family.  This protein catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide.  Loss of function or expression of this gene is associated with decreased apoptosis, increased cell motility, and cancer progression. Aldh1l1 is cytoplasmic. Diseases associated with ALDH1L1 include Methanol Poisoning and Pilocytic Astrocytoma.  Anti-Aldh1l1 Antibody is useful for researchers interested in Apoptosis Research, Metabolism Research, and Cancer research.

Gene ID: 107747

NCBI Accession: NP_081682

UniProt: Q8R0Y6