ASCL1 antibody (Internal Region)

Catalog No. ABIN7540559

Supplier Product No.: 600-101-mh4

This Goat Polyclonal antibody specifically detects ASCL1 in WB, ELISA and IHC. It exhibits reactivity toward Mouse.

Quick Overview for ASCL1 antibody (Internal Region) (ABIN7540559)

Target: ASCL1 (Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This ASCL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-ASCL1 Antibody

Binding Specificity: Internal Region

Supplier Product No.: 600-101-mh4

Supplier: Rockland

Purpose: Mash1/Ascl1 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against mouse Mash1/Ascl1.

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-Mash1/Ascl1 antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of mouse Mash1/Ascl1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000-1:50,000

Immunohistochemistry_Dilution: 1:1000

Western_Blot_Dilution: 1:1000

Comment:

Anti-Mash1/Ascl1 Antibody has been tested in Western Blot and Immunohistochemistry. Expect a band at ~24.7 kDa in western blot using appropriate lysates or tissues. Positive control used was SH-SY5Y whole cell lysate in WB, human thyroid papillary carcinoma tissue in IHC.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for ASCL1

Target: ASCL1 (Achaete-Scute Complex Homolog 1 (Drosophila) (ASCL1))

Alternative Name: Ascl1

Background: Goat Anti-Ascl1 Antibody, Goat Anti-Mash1 Antibody, Achaete-scute homolog 1, ASH-1, mASH-1,Ascl1 (Achaete-scute homolog 1) is a transcription factor that plays a key role in neuronal differentiation, it acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways. It directly binds the E box motif (5'-CANNTG-3') on promoters and promotes transcription of neuronal genes. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Ascl1 plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. It is essential for the generation of olfactory and autonomic neurons. It acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Anti-Mash1/Ascl1 Antibody is useful for researchers interested in CCHS, SIDS, Dopaminergic Neurogenesis, and DNA binding.

Gene ID: 17172

NCBI Accession: NP_032579

UniProt: Q02067

Pathways: Dopaminergic Neurogenesis