Doublecortin antibody (C-Term)

Catalog No. ABIN7540562

Supplier Product No.: 600-101-mh8s

This Goat Polyclonal antibody specifically detects Doublecortin in WB, ELISA, IHC, IF and FM. It exhibits reactivity toward Human.

Quick Overview for Doublecortin antibody (C-Term) (ABIN7540562)

Target: Doublecortin (DCX)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Doublecortin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Product Details anti-Doublecortin Antibody

Binding Specificity: C-Term

Supplier Product No.: 600-101-mh8s

Supplier: Rockland

Purpose: Doublecortin Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human Neuronal migration protein doublecortin.

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-Doublecortin antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a near C-terminal portion of human doublecortin conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000-1:50,000

Immunohistochemistry_Dilution: 1:200

IF_Microscopy_Dilution: 15 μg/mL

Western_Blot_Dilution: 1:1000

Comment:

Anti-Doublecortin Antibody has been tested in WB, IF, and IHC.  Expect bands ~40.6 kDa in western blot using appropriate lysates.  Positive control used: PND2-6 brain cells in western blot, PND1 in immunofluorescence, and mouse dentate gyrus for immunohistochemistry.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Doublecortin

Target: Doublecortin (DCX)

Alternative Name: DCX

Background: Goat Anti-Doublecortin Antibody, Lissencephalin-X, Doublecortex,  Doublin, Lis-X, DBCN, LISX, Doublecortex Lissencephaly X-Linked (Doublecortin), Neuronal Migration Protein Doublecortin, SCLH, XLIS, DC,DCX (Neuronal Migration Protein Doublecortin) is a microtubule-associated protein required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. It may act by competing with the putative neuronal protein kinase DCLK1 in binding to a target protein. In that way, it may participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. Doublecortin may be part with PAFAH1B1/LIS-1 of overlapping, but distinct, signaling pathways that promote neuronal migration. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, cognitive disability, subcortical band heterotopia ("double cortex" syndrome) in females and lissencephaly ("smooth brain" syndrome) in males. Anti-Neuronal Migration Protein Doublecortin Antibody is useful for researcher interested in developmental biology, cytoskeletal signaling, neuroscience, and protein kinase binding.

Gene ID: 1641

NCBI Accession: NP_000546

UniProt: O43602