PIK3R3 antibody (N-Term)
Quick Overview for PIK3R3 antibody (N-Term) (ABIN7540566)
Target
See all PIK3R3 AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- N-Term
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Supplier Product No.
- 600-101-ml2s
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Supplier
- Rockland
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Purpose
- Anti-PI3 Kinase p55 gamma Antibody
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Cross-Reactivity (Details)
- This affinity purified antibody is directed against human PI 3 Kinase p55 gamma.
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Purification
- The product was affinity purified from monospecific antiserum by immunoaffinity purification.
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Sterility
- Sterile filtered
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Immunogen
- Anti-PI3 Kinase p55 gamma antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a near N-Terminal portion of human PI 3 Kinase p55 gamma conjugated to Keyhole Limpet Hemocyanin (KLH).
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Isotype
- IgG
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Application Notes
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ELISA_Dilution: 1:10,000 - 50,000
Immunohistochemistry_Dilution: 1:100
IF_Microscopy_Dilution: 15 μg/mL
Western_Blot_Dilution: 1:1000
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Comment
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Anti-PI3 Kinase p55 gamma Antibody has been tested in WB, IF, and IHC. Expect bands ~44, 47, 54kDa in western blot using appropriate tissues or lysates. Positive control used: mouse testis in WB, A431 cells in IF, Human testis and Human tonsil tissue in IHC.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide -
Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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Expiry Date
- 12 months
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- PIK3R3 (Phosphatidylinositol 3-kinase regulatory subunit gamma (PIK3R3))
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Alternative Name
- PIK3R3
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Background
- Goat Anti-PI3 Kinase p55 gamma Antibody, Goat Anti-PIK3R3 Antibody, Anti-p55 gamma Antibody, Phosphoinositide-3-Kinase Regulatory Subunit 3, Phosphatidylinositol 3-Kinase 55 KDa Regulatory Subunit Gamma, Phosphoinositide-3-Kinase, Regulatory Subunit 3 (Gamma), PtdIns-3-Kinase Regulatory Subunit P55-Gamma, PI3-Kinase Regulatory Subunit Gamma, PI3K Regulatory Subunit Gamma, PI3-Kinase Subunit P55-Gamma, P55PIK, Phosphatidylinositol 3-Kinase, Regulatory Subunit, Polypeptide 3 (P55, Gamma), Phosphatidylinositol 3-Kinase Regulatory Subunit Gamma, Phosphoinositide-3-Kinase Regulatory Subunit Gamma, PtdIns-3-Kinase Regulatory Subunit Gamma, P55-GAMMA, P55,PI 3-Kinases (phosphoinositide 3-kinases, PI 3-Ks) are a family of lipid kinases capable of phosphorylating the 3'OH of the inositol ring of phosphoinositides. They are responsible for coordinating a diverse range of cell functions including proliferation and survival. PIK3R3 (Phosphoinositide-3-Kinase Regulatory Subunit 3) binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1. Anti-PIK3R3 Antibody is useful for researchers interested in cancer research, Asbestos-related lung carcinomas, intravenous Leiomyomatosis, ERK signaling, and mTOR Pathways.
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Gene ID
- 8503
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NCBI Accession
- NP_001107644
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UniProt
- Q92569
Target
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