OPN1SW antibody (N-Term)

Catalog No. ABIN7540578

Supplier Product No.: 600-101-mp7

The Goat Polyclonal anti-OPN1SW antibody has been validated for ELISA and IHC. It is suitable to detect OPN1SW in samples from Mouse. There are 2+ publications available.

Quick Overview for OPN1SW antibody (N-Term) (ABIN7540578)

Target: OPN1SW (Opsin 1 (Cone Pigments), Short-Wave-Sensitive (OPN1SW))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This OPN1SW antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Product Details anti-OPN1SW Antibody

Binding Specificity: N-Term

Supplier Product No.: 600-101-mp7

Supplier: Rockland

Purpose: S Opsin Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against mouse S Ospin.

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-S Opsin antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a N-terminal portion of mouse S Opsin conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000 - 1:50,000

Immunohistochemistry_Dilution: 1:100

Comment:

Suggested Applications:

Suggested_Applications: IF

Other_Performance_Data: The 1:100 H1-20 provides optimal staining with staining of structures in multiple layers. These layers have been shown to express S-opsin in the cited reference. For staining only in the outer segment layer, the 1:200 and 1:300 H2-20 appears to be ideal.

Anti-S Ospin Antibody has been tested in ELISA and IHC.  Expect a band at ~39kDa in western blot using appropriate tissues and lysates.  Positive control used: Mouse eye tissue in Immunohistochemistry.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-OPN1SW antibody (ABIN7540578)

Gupta, Młynarski, Sumser, Symonova, Svatoň, Joesch: "Panoramic visual statistics shape retina-wide organization of receptive fields." in: Nature neuroscience, Vol. 26, Issue 4, pp. 606-614, (2023) (PubMed).

Simons, Smith, Dengler-Crish, Crish: "Retinal ganglion cell loss and gliosis in the retinofugal projection following intravitreal exposure to amyloid-beta." in: Neurobiology of disease, Vol. 147, pp. 105146, (2021) (PubMed).

Target Details for OPN1SW

Target: OPN1SW (Opsin 1 (Cone Pigments), Short-Wave-Sensitive (OPN1SW))

Alternative Name: Opn1sw

Background: Goat Anti-Short-wave-sensitive opsin 1 Antibody, S opsin, Blue cone photoreceptor pigment, Blue-sensitive opsin, BOP, Short wavelength-sensitive cone opsin, Bcp, Opn1sw,Short-wave-sensitive opsin 1 belongs to the G-protein coupled receptor 1 family, opsin subfamily. It encodes the blue cone pigment gene which is one of three types of cone photoreceptors responsible for normal color vision. Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal. Required for the maintenance of cone outer segment organization in the ventral retina, but not essential for the maintenance of functioning cone photoreceptors. Involved in ensuring correct abundance and localization of retinal membrane proteins. May increase spectral sensitivity in dim light. Defects in this gene are the cause of tritan color blindness (tritanopia). Affected individuals lack blue and yellow sensory mechanisms while retaining those for red and green. Defective blue vision is characteristic. Anti-S Ospin Antibody is useful for researchers interested in Color Blindness, Metabolism of fat-soluble vitamins, and Peptide ligand-binding receptors.

Gene ID: 12057

NCBI Accession: NP_031564

UniProt: P51491