SLC40A1 antibody (Internal Region)

Catalog No. ABIN7540602

Supplier Product No.: 600-401-mg1s

The Rabbit Polyclonal anti-SLC40A1 antibody is suitable to detect SLC40A1 in samples from Human. It has been validated for WB, ELISA, IF and FM.

Quick Overview for SLC40A1 antibody (Internal Region) (ABIN7540602)

Target: SLC40A1 (Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC40A1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Product Details anti-SLC40A1 Antibody

Binding Specificity: Internal Region

Supplier Product No.: 600-401-mg1s

Supplier: Rockland

Purpose: SLC40A1 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human SLC40A1.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-SLC40A1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human SLC40A1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000 - 1:50,000

IF_Microscopy_Dilution: 15 μg/mL

Western_Blot_Dilution: 10 μg/mL

Comment:

Anti-SLC40A1 Antibody has been tested in ELISA, WB, and IF.  Expect a band at ~62.5kDa in western blot using appropriate lysates.  Positive control used: Caco-2 lysate in Western Blot and Hep-G2 in Immunofluorescence.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for SLC40A1

Target: SLC40A1 (Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1 (SLC40A1))

Alternative Name: SLC40A1

Background: Rabbit Anti-SLC40A1 Antibody, Solute Carrier Family 40 Member 1, Solute Carrier Family 11 (Proton-Coupled Divalent Metal Ion Transporters), Member 3, Solute Carrier Family 40 (Iron-Regulated Transporter), Member 1, SLC11A3, IREG1, FPN1, Iron-Regulated Transporter 1, Iron Regulated Gene 1, Ferroportin-1, Ferroportin 1, MSTP079, MST079, HFE4, MTP1, SLC40A1 (Solute Carrier Family 40 Member 1) may be involved in iron export from duodenal epithelial cell and in transfer of iron between maternal and fetal circulation. Mediates iron efflux in the presence of a ferroxidase (hephaestin and/or ceruloplasmin).  Defects in this gene are a cause of hemochromatosis type 4 (HFE4). Diseases associated with SLC40A1 include Hemochromatosis Type 4 and Type 1.  Anti-SLC40A1 Antibody is useful for researchers interested in mineral absorption, insulin receptor recycling, and transmembrane transporter activity.

Gene ID: 30061

NCBI Accession: NP_055400

UniProt: Q9NP59

Pathways: Transition Metal Ion Homeostasis