Caspase 12 antibody (Internal Region)

Catalog No. ABIN7540611

Supplier Product No.: 600-401-mj3

The Rabbit Polyclonal anti-Caspase 12 antibody has been validated for WB, ELISA, IF and FM. It is suitable to detect Caspase 12 in samples from Mouse.

Quick Overview for Caspase 12 antibody (Internal Region) (ABIN7540611)

Target: Caspase 12 (CASP12) (Caspase 12 (Gene/pseudogene) (CASP12))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 12 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Product Details anti-Caspase 12 Antibody

Binding Specificity: Internal Region

Supplier Product No.: 600-401-mj3

Supplier: Rockland

Purpose: Caspase 12 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against mouse Caspase 12.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-Caspase 12 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of mouse Caspase 12 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000 - 1:50,000

IF_Microscopy_Dilution: 15 μg/mL

Western_Blot_Dilution: 5 μg/mL

Comment:

Anti-Caspase 12 Antibody has been tested in ELISA, IF, and WB.  Positive control used: NIH/3T3 and PC-12 lysates in Western Blot and NIH/3T3 in Immunofluorescence.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Caspase 12

Target: Caspase 12 (CASP12) (Caspase 12 (Gene/pseudogene) (CASP12))

Alternative Name: Casp12

Background: Rabbit Anti-Caspase 12 Antibody, Caspase 12 pseudogene 1, Casp12, Casp-12, CASP12P1,Casp12 (Caspase 12 (Gene/Pseudogene)) has no protease activity. It may reduce cytokine release in response to bacterial lipopolysaccharide during infections. Caspase 12 reduces activation of NF-kappa-B in response to TNF. Caspases (cysteinyl aspartate proteases) are involved in the signaling pathways of apoptosis, necrosis and inflammation. These enzymes can be divided into initiators and effectors. The initiator isoforms are activated by, and interact with, upstream adaptor molecules. Anti-Caspase 12 Antibody is useful for researchers interested in Amyotrophic lateral sclerosis (ALS), ATP synthesis, and Apoptosis research.

Gene ID: 12364

NCBI Accession: NP_033938

UniProt: O08736

Pathways: Apoptosis, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response