FOXP3 antibody (N-Term)

Catalog No. ABIN7540626

Supplier Product No.: 600-401-mn0s

The Rabbit Polyclonal anti-FOXP3 antibody has been validated for WB, FACS, ELISA, IF, FM and MA. It is suitable to detect FOXP3 in samples from Mouse.

Quick Overview for FOXP3 antibody (N-Term) (ABIN7540626)

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXP3 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM), Multiplex Assay (MA)

Product Details anti-FOXP3 Antibody

Binding Specificity: N-Term

Supplier Product No.: 600-401-mn0s

Supplier: Rockland

Purpose: Foxp3 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against mouse Foxp3.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-Foxp3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a near N-terminal portion of mouse Foxp3 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000-1:50,000

Flow_Cytometry_Dilution: 1:25

IF_Microscopy_Dilution: 15 μg/mL

Western_Blot_Dilution: 1:1000

Comment:

Anti-Foxp3 Antibody has been tested in ELISA, WB, IF, and FLOW.  Expect a band for isoforms 1 and 4 at ~47.2 and 44.4kDa in western blot using appropriate tissues and lysates.  Positive control used: NIH/3T3 cells in Immunofluorescence and Flow cytometry.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for FOXP3

Target: FOXP3 (Forkhead Box P3 (FOXP3))

Alternative Name: Foxp3

Background: Rabbit Anti-Forkhead Box P3 Antibody, Immune Dysregulation Polyendocrinopathy Enteropathy, X-Linked, Forkhead Box Protein P3, Scurfin, IPEX, Immunodeficiency Polyendocrinopathy Enteropathy X-Linked, FOXP3delta7, DIETER, AIID, PIDX, XPID, JM2, Forkhead box protein P3 41 kDa form, Forkhead box protein P3 C-terminally processed,FOXP3 is a member of the forkhead/winged-helix family of transcriptional regulators. The FOX family of transcription factors is a large group of proteins that share a common DNA binding domain termed a winged-helix or forkhead domain. FOXP3 acts as a repressor of transcription and regulates T cell activation, with its overexpression in CD4 T cells leading to an attenuation of activation-induced cytokine production and proliferation. In regulatory T (Treg) cells, FOXP3 is essential for Treg suppressor function and its expression leads to the repression of IL-17 expression. Mutations in FOXP3 gene cause IPEX, a fatal, X-linked inherited disorder characterized by immune dysregulation. The FOXP3 protein is essential for normal immune homeostasis. Specifically, FOXP3 represses transcription through a DNA binding forkhead domain, thereby regulating T cell activation. Anti-FOXP3 antibody is useful for researchers interested in transcription factor activity, polyendocrinopathy, immunodysregulation, and DNA binding research.

Gene ID: 20371

NCBI Accession: NP_001186276

UniProt: Q99JB6

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation