SMAD2 antibody (pSer467)

Catalog No. ABIN7540643

Supplier Product No.: 600-401-mr3s

This Rabbit Polyclonal antibody specifically detects SMAD2 in WB, ELISA, IHC, IF, DB and FM. It exhibits reactivity toward Human.

Quick Overview for SMAD2 antibody (pSer467) (ABIN7540643)

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Dot Blot (DB), Fluorescence Microscopy (FM)

Product Details anti-SMAD2 Antibody

Binding Specificity: pSer467

Supplier Product No.: 600-401-mr3s

Supplier: Rockland

Purpose: SMAD2 phospho S467 Antibody

Cross-Reactivity (Details): Anti-SMAD2 pS467 antibody is directed against the phosphorylated form of human Smad2 protein at the pS467 residue.

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: SMAD2 pS467 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-terminal of human SMAD2 protein.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1.0 μg/mL

Immunohistochemistry_Dilution: 1:2000

IF_Microscopy_Dilution: 15 μg/mL

Western_Blot_Dilution: User Optimized

Other: Dot Blot: 1 μg/mL

Comment:

Anti-SMAD2 pS467 affinity purified antibody has been tested for use in ELISA, DB, IHC, and IF.  Expect a band approximately ~49, 52.3 kDa in size corresponding to human Smad2 protein by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. Positive controls used: HeLa cells in Immunofluorescence and Human brain cortex in Immunohistochemistry.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for SMAD2

Target: SMAD2 (SMAD, Mothers Against DPP Homolog 2 (SMAD2))

Alternative Name: SMAD2

Background: Rabbit Anti-SMAD2 pS467 antibody, SMAD-2, SMAD 2, mothers against decapentaplegic homolog 2 antibody, MAD homolog 2, Mothers against DPP homolog 2, SMAD family member 2, MADH2, MADH 2, JV18-1,Smad2 (Mothers against decapentaplegic homolog 2) is a member of the Smad family of proteins which are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. Anti-SMAD2 pS467 antibody is ideal for researchers interested in Cancer, Immunology and Nuclear Signaling research.

Gene ID: 4087

NCBI Accession: NP_001003652

UniProt: Q15796

Pathways: Cell Division Cycle, Hormone Transport, Chromatin Binding, Protein targeting to Nucleus