SARS-CoV-2 Nucleocapsid antibody

Catalog No. ABIN7540644

Supplier Product No.: 600-401-ms4

The Rabbit Polyclonal anti-SARS-CoV-2 Nucleocapsid antibody has been validated for ELISA and WB. It is suitable to detect SARS-CoV-2 Nucleocapsid in samples from SARS Coronavirus-2 (SARS-CoV-2). There is 1 publication available.

Quick Overview for SARS-CoV-2 Nucleocapsid antibody (ABIN7540644)

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Reactivity: SARS Coronavirus-2 (SARS-CoV-2)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SARS-CoV-2 Nucleocapsid antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-SARS-CoV-2 Nucleocapsid Antibody

Supplier Product No.: 600-401-ms4

Supplier: Rockland

Purpose: SARS-CoV-2 Nucleocapsid (N) Protein Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against SARS Coronavirus 2 Nucleocapsid (N) protein

Purification: The product was purified from monospecific antiserum by immunoaffinity chromatography over SARS CoV-2 resin.

Sterility: Sterile filtered

Immunogen: Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody was produced by repeated immunizations with purified recombinant SARS-CoV-2 Nucleocapsid protein with C-terminal His-tag, derived from the transfected human HEK293 cells.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:500,000-1:600,000

Western_Blot_Dilution: User Optimized

Comment:

Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody has been tested for use in ELISA and is suitable in Western Blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 47 kDa in size corresponding to SARS-CoV-2 Nucleocapsid (N) protein by western blotting in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-SARS-CoV-2 Nucleocapsid antibody (ABIN7540644)

Wang, Wang, Gupta, Morrissey, Naik, Singamaneni: "Enhancing the Stability of COVID-19 Serological Assay through Metal-Organic Framework Encapsulation." in: Advanced healthcare materials, Vol. 10, Issue 18, pp. e2100410, (2021) (PubMed).

Target Details for SARS-CoV-2 Nucleocapsid

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Alternative Name: SARS-CoV-2 Nucleocapsid

Target Type: Viral Protein

Background: Rabbit anti-SARS CoV 2 Nucleocapsid Protein Antibody, N-protein antibody, SARS CoV2 antibody, 2019-nCoV, COVID-19, Severe acute respiratory syndrome antibody, Severe acute respiratory syndrome coronavirus 2,SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2 or COVID-19) is related to SARS-CoV, MERS, and four milder coronaviruses (HKU1, NL63, OC43 and 229E). SARS-CoV-2 is an enveloped positive-strand RNA virus that consists of four structural proteins: spike (S) protein, envelope (E) protein, membrane (M) protein and nucleocapsid (N) protein. The spike protein is the most important surface protein of coronavirus. SARS-CoV-2 has a high affinity binding to human receptor ACE2 (angiotensin-converting enzyme 2) within respiratory epithelial. ACE2 is a membrane-bound aminopeptidase that has a vital role in the cardiovascular and immune systems. The nucleocapsid protein is a most abundant protein of coronavirus. The coronavirus nucleocapsid protein is the major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid. Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody is useful for researchers interested in diagnostics and viral research.

Gene ID: 43740575

UniProt: P0DTC9