SARS-CoV-2 Nucleocapsid antibody

Catalog No. ABIN7540645

Supplier Product No.: 600-401-ms4s

This anti-SARS-CoV-2 Nucleocapsid antibody is a Rabbit Polyclonal antibody detecting SARS-CoV-2 Nucleocapsid in ELISA and WB. Suitable for SARS Coronavirus-2 (SARS-CoV-2). This Primary Antibody has been cited in 1 publication.

Quick Overview for SARS-CoV-2 Nucleocapsid antibody (ABIN7540645)

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Reactivity: SARS Coronavirus-2 (SARS-CoV-2)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SARS-CoV-2 Nucleocapsid antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-SARS-CoV-2 Nucleocapsid Antibody

Supplier Product No.: 600-401-ms4s

Supplier: Rockland

Purpose: SARS-CoV-2 Nucleocapsid (N) Protein Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against SARS Coronavirus 2 Nucleocapsid (N) protein.

Purification: The product was purified from monospecific antiserum by immunoaffinity chromatography over SARS CoV-2 resin.

Sterility: Sterile filtered

Immunogen: Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody was produced by repeated immunizations with purified recombinant SARS-CoV-2 Nucleocapsid protein with C-terminal His-tag, derived from the transfected human HEK293 cells.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:500,000 - 1:600,000

Western_Blot_Dilution: User Optimized

Comment:

Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody has been tested for use in ELISA and is suitable in Western Blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 47 kDa in size corresponding to SARS-CoV-2 Nucleocapsid (N) protein by western blotting in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

References for anti-SARS-CoV-2 Nucleocapsid antibody (ABIN7540645)

Wang, Wang, Gupta, Morrissey, Naik, Singamaneni: "Enhancing the Stability of COVID-19 Serological Assay through Metal-Organic Framework Encapsulation." in: Advanced healthcare materials, Vol. 10, Issue 18, pp. e2100410, (2021) (PubMed).

Target Details for SARS-CoV-2 Nucleocapsid

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Alternative Name: SARS-CoV-2 Nucleocapsid

Target Type: Viral Protein

Background: Rabbit anti-SARS CoV 2 Nucleocapsid Protein Antibody, N-protein antibody, SARS CoV2 antibody, 2019-nCoV, COVID-19, Severe acute respiratory syndrome antibody, Severe acute respiratory syndrome coronavirus 2,SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2 or COVID-19) is related to SARS-CoV, MERS, and four milder coronaviruses (HKU1, NL63, OC43 and 229E). SARS-CoV-2 is an enveloped positive-strand RNA virus that consists of four structural proteins: spike (S) protein, envelope (E) protein, membrane (M) protein and nucleocapsid (N) protein. The spike protein is the most important surface protein of coronavirus. SARS-CoV-2 has a high affinity binding to human receptor ACE2 (angiotensin-converting enzyme 2) within respiratory epithelial. ACE2 is a membrane-bound aminopeptidase that has a vital role in the cardiovascular and immune systems. The nucleocapsid protein is a most abundant protein of coronavirus. The coronavirus nucleocapsid protein is the major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid. Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody is useful for researchers interested in diagnostics and viral research.

Gene ID: 43740575

UniProt: P0DTC9