SARS-CoV-2 Nucleocapsid antibody (Biotin)

Catalog No. ABIN7540650

Supplier Product No.: 600-406-ms4

The Rabbit Polyclonal anti-SARS-CoV-2 Nucleocapsid antibody has been validated for ELISA and WB. It is suitable to detect SARS-CoV-2 Nucleocapsid in samples from SARS Coronavirus-2 (SARS-CoV-2).

Quick Overview for SARS-CoV-2 Nucleocapsid antibody (Biotin) (ABIN7540650)

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Reactivity: SARS Coronavirus-2 (SARS-CoV-2)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SARS-CoV-2 Nucleocapsid antibody is conjugated to Biotin

Application: ELISA, Western Blotting (WB)

Product Details anti-SARS-CoV-2 Nucleocapsid Antibody (Biotin)

Supplier Product No.: 600-406-ms4

Supplier: Rockland

Purpose: SARS-CoV-2 Nucleocapsid (N) Protein Antibody Biotin Conjugated

Cross-Reactivity (Details): This affinity purified conjugated antibody is directed against SARS Coronavirus 2 Nucleocapsid (N) protein.

Purification: The product was purified from monospecific antiserum by immunoaffinity chromatography over SARS CoV-2 resin.

Immunogen: Anti-SARS-CoV-2 Nucleocapsid (N) Protein Biotin Conjugated Antibody was produced by repeated immunizations with purified recombinant SARS-CoV-2 Nucleocapsid protein with C-terminal His-tag, derived from the transfected human HEK293 cells.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:350,000-1:450,000

Western_Blot_Dilution: User Optimized

Comment:

Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody conjugated to biotin has been tested for use in ELISA.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 47 kDa in size corresponding to SARS-CoV-2 Nucleocapsid (N) protein by western blotting in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for SARS-CoV-2 Nucleocapsid

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Alternative Name: SARS-CoV-2 Nucleocapsid

Target Type: Viral Protein

Background: Rabbit anti-SARS CoV 2 Nucleocapsid Protein Antibody, N-protein antibody, SARS CoV2 antibody, 2019-nCoV, COVID-19, Severe acute respiratory syndrome antibody, Severe acute respiratory syndrome coronavirus 2,SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2) the virus that causes the disease COVID-19, is related to SARS-CoV, MERS-CoV, and other betacoronaviruses (HKU1, NL63, OC43 and 229E). SARS-CoV-2 is an enveloped positive strand RNA virus that consists of several structural proteins including spike (S) protein, envelope (E) protein, membrane (M) protein and nucleocapsid (N) protein, and other non-structural proteins (NSPs). The coronavirus nucleocapsid protein is the major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid. Sequence comparison of the N genes of five strains of the coronavirus mouse hepatitis virus suggests a three-domain structure for the nucleocapsid protein. Anti-SARS-CoV Nucleocapsid (N) Protein Antibody is useful for researchers interested in diagnostics, and viral research.

Gene ID: 43740575

UniProt: P0DTC9