SARS-CoV-2 NSP8 antibody

Catalog No. ABIN7540672

Supplier Product No.: 100-401-a53

This anti-SARS-CoV-2 NSP8 antibody is a Rabbit Polyclonal antibody detecting SARS-CoV-2 NSP8 in WB, IF, FM and IP. Suitable for SARS Coronavirus (SARS-CoV). This Primary Antibody has been cited in 1 publication.

Quick Overview for SARS-CoV-2 NSP8 antibody (ABIN7540672)

Target: SARS-CoV-2 NSP8 (NSP8) (SARS-CoV-2 Non-Structural Protein 8 (NSP8))

Reactivity: SARS Coronavirus (SARS-CoV)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SARS-CoV-2 NSP8 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Fluorescence Microscopy (FM), Immunoprecipitation (IP)

Product Details anti-SARS-CoV-2 NSP8 Antibody

Supplier Product No.: 100-401-a53

Supplier: Rockland

Purpose: Sars-Cov Nonstructural Protein 8 Antibody

Cross-Reactivity (Details): This antibody is directed against SARS-Coronavirus nsp8 protein.

Purification: The product is neat antiserum.

Sterility: Sterile filtered

Immunogen: This whole rabbit serum was produced by repeated immunizations with a purified His- tagged recombinant protein corresponding to full-length SARS-Coronavirus nsp8.

Isotype: IgG

Application Details

Application Notes:

Immunoprecipitation_Dilution: 1:60

IF_Microscopy_Dilution: 1:300

Western_Blot_Dilution: 1:1,000

Other: IMMUNOELECTRON MICROSCOPY 1:100

Comment:

This antibody has been tested for use in immunofluorescence microscopy, immunoelectron microscopy, immunoprecipitation and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 22 kDa in size corresponding to SARS-CoV nsp8 by western blotting in the appropriate cell lysate or extract. For immunofluorescence microscopy, Vero-E6 cells, grown on glass slides, were infected with SARS-CoV-Fr1 strain for 1 h at 37°C.  Infection occurred in PBS/DEAE/2% FCS followed by exchange to EMEM/25mM HEPES/2% FCS. Cells were fixed with PBS/3% PFA.  After washing fixed cells, antibody incubation was performed in PBS/5% FCS for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: None

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-SARS-CoV-2 NSP8 antibody (ABIN7540672)

Shi, Yu, Li, Cui, Zhao, Wang, Wang, Zhang, Huang: "Expression Profile and Localization of SARS-CoV-2 Nonstructural Replicase Proteins in Infected Cells." in: Microbiology spectrum, Vol. 10, Issue 4, pp. e0074422, (2022) (PubMed).

Target Details for SARS-CoV-2 NSP8

Target: SARS-CoV-2 NSP8 (NSP8) (SARS-CoV-2 Non-Structural Protein 8 (NSP8))

Alternative Name: SARS-CoV-2 NSP8

Background: Rabbit anti-Sars-Cov Nonstructural Protein 8 Antibody, Replicase polyprotein 1a, ORF1a polyprotein, nsp8,The nonstructural protein 8 (nsp8) is one of the SARS-Coronavirus replicase cleaving products, encoded by ORF1a. Nsp8 is thought to be part of the viral replication complex, which is associated with intracellular membranes.  No specific information on the function of nsp8 is available. Anti-SARS-CoV Nonstructural Protein 8 (nsp8) Antibody is useful for researchers interested in viral research.

Gene ID: 1489680, 30124074

UniProt: P0C6U8