SARS-Coronavirus Nucleocapsid Protein (SARS-CoV N) antibody

Catalog No. ABIN7540673

Supplier Product No.: 200-301-ms4

This Mouse Monoclonal antibody specifically detects in ELISA. It exhibits reactivity toward SARS Coronavirus-2 (SARS-CoV-2).

Quick Overview for SARS-Coronavirus Nucleocapsid Protein (SARS-CoV N) antibody (ABIN7540673)

Target: SARS-Coronavirus Nucleocapsid Protein (SARS-CoV N)

Reactivity: SARS Coronavirus-2 (SARS-CoV-2)

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA

Clone: 7G9-C2

Product Details

Supplier Product No.: 200-301-ms4

Supplier: Rockland

Purpose: SARS-CoV-2 Nucleocapsid (N) Protein Antibody

Cross-Reactivity (Details): Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody is directed against SARS Coronavirus 2 Nucleocapsid (N) protein.

Purification: The product was purified from roller bottle culture by protein A chromatography.

Sterility: Sterile filtered

Immunogen: This protein A purified antibody was produced by repeated immunizations with purified recombinant SARS-CoV-2 Nucleocapsid protein with C-terminal His-tag, derived from the transfected human HEK293 cells.

Isotype: IgG1 kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Anti-SARS-CoV-2 Nucleocapsid (N) Protein Monoclonal Antibody has been tested for use in ELISA.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 47 kDa in size corresponding to SARS-CoV-2 Nucleocapsid (N) protein by western blotting in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: SARS-Coronavirus Nucleocapsid Protein (SARS-CoV N)

Alternative Name: SARS-CoV Nucleocapsid

Background: Mouse anti-SARS CoV 2 Nucleocapsid Protein Antibody, N-protein antibody, SARS CoV2 antibody, 2019-nCoV, COVID-19, Severe acute respiratory syndrome antibody, Severe acute respiratory syndrome coronavirus 2,SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2) the virus that causes the disease COVID-19, is related to SARS-CoV, MERS-CoV, and other betacoronaviruses (HKU1, NL63, OC43 and 229E). SARS-CoV-2 is an enveloped positive strand RNA virus that consists of several structural proteins including spike (S) protein, envelope (E) protein, membrane (M) protein and nucleocapsid (N) protein, and other non-structural proteins (NSPs). The coronavirus nucleocapsid protein is the major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid. Sequence comparison of the N genes of five strains of the coronavirus mouse hepatitis virus suggests a three-domain structure for the nucleocapsid protein. Anti-SARS-CoV Nucleocapsid (N) Protein Antibody is useful for researchers interested in diagnostics and viral research.

Gene ID: 43740575

UniProt: P0DTC9