SARS-Coronavirus Nucleocapsid Protein (SARS-CoV N) antibody

Catalog No. ABIN7540675

Supplier Product No.: 200-401-ms4-0100

This anti- antibody is a Rabbit Polyclonal antibody detecting in ELISA and WB. Suitable for SARS Coronavirus-2 (SARS-CoV-2).

Quick Overview for SARS-Coronavirus Nucleocapsid Protein (SARS-CoV N) antibody (ABIN7540675)

Target: SARS-Coronavirus Nucleocapsid Protein (SARS-CoV N)

Reactivity: SARS Coronavirus-2 (SARS-CoV-2)

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details

Supplier Product No.: 200-401-ms4-0100

Supplier: Rockland

Purpose: SARS-CoV-2 Nucleocapsid (N) Protein Antibody

Cross-Reactivity (Details): This protein A purified antibody is directed against SARS Coronavirus 2 Nucleocapsid (N) protein.

Purification: The product was purified from monospecific antiserum by protein A affinity purification.

Immunogen: Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody was produced by repeated immunizations with purified recombinant SARS-CoV-2 Nucleocapsid protein with C-terminal His-tag, derived from the transfected human HEK293 cells.

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: User Optimized

Western_Blot_Dilution: 1:1000-1:2000

Comment:

Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody has been tested for use in ELISA and Western Blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 47 kDa in size corresponding to SARS-CoV-2 Nucleocapsid (N) protein by western blotting in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution_Buffer: Restore with deionized water (or equivalent)

Reconstitution_Volume: 100 μL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: SARS-Coronavirus Nucleocapsid Protein (SARS-CoV N)

Alternative Name: SARS-CoV Nucleocapsid

Background: Rabbit anti-SARS CoV 2 Nucleocapsid Protein Antibody, N-protein antibody, SARS CoV2 antibody, 2019-nCoV, COVID-19, Severe acute respiratory syndrome antibody, Severe acute respiratory syndrome coronavirus 2,SARS-CoV-2 (Severe acute respiratory syndrome coronavirus 2 or COVID-19) is related to SARS-CoV, MERS, and four milder coronaviruses (HKU1, NL63, OC43 and 229E). SARS-CoV-2 is an enveloped positive-strand RNA virus that consists of four structural proteins: spike (S) protein, envelope (E) protein, membrane (M) protein and nucleocapsid (N) protein. The spike protein is the most important surface protein of coronavirus. SARS-CoV-2 has a high affinity binding to human receptor ACE2 (angiotensin-converting enzyme 2) within respiratory epithelial. ACE2 is a membrane-bound aminopeptidase that has a vital role in the cardiovascular and immune systems. The nucleocapsid protein is a most abundant protein of coronavirus. The coronavirus nucleocapsid protein is the major structural component of virions that associates with genomic RNA to form a long, flexible, helical nucleocapsid. Anti-SARS-CoV-2 Nucleocapsid (N) Protein Antibody is useful for researchers interested in diagnostics and viral research.

Gene ID: 43740575

UniProt: P0DTC9