Recombinant CA9 antibody

Catalog No. ABIN7542959

This Mouse Monoclonal antibody specifically detects CA9 in WB, IHC, ELISA, IF, FACS, BP and Crys. It exhibits reactivity toward Human.

Quick Overview for Recombinant CA9 antibody (ABIN7542959)

Target: CA9 (Carbonic Anhydrase IX (CA9))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CA9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Blocking Peptide (BP), Crystallization (Crys)

Clone: M75

Product Details anti-CA9 Antibody

Purpose: Anti-Carbonic Anhydrase IX [M75], Mouse IgG1, kappa

Specificity: This antibody is specific for the sequence PGEEDLP, which is repeated several times between the 61st and 96th aa residues of human carbonic anhydrase IX's PG domain.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/C mice with MaTu-infected HeLa cells.

Isotype: IgG1 kappa

Application Details

Application Notes: The original format of this antibody (mouse IgG2b) was characterized by and used in IB (WB), IF, and FC assays (Pastoreková et al., 1992, PMID: 1312272). This antibody's epitope has been determined by a competitive ELISA against the peptide APGEEDLPA. Furthermore, it was used for WB and was shown to blocks the capacity of immobilized MN/CA IX to attach cells (Závada et al., 2000, PMID: 10839295). The Fab version of this antibody was crystallized and analyzed. Additionally, an scFv version of this antibody was created, and its thermodynamic parameters against the peptide DLPGEEDLPGEE were evaluated via ITC. Its K_d was determined to be 0.42 μM (Král et al., 2008, PMID: 18041760). This antibody was used for WB in breast cell lines and for IHC in xenograft tumors grown from PC-3 cells in mice (Li et al., 2009, PMID: 19538935).

Comment:

This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CA9

Target: CA9 (Carbonic Anhydrase IX (CA9))

Alternative Name: Carbonic Anhydrase IX

Background: CA9, CAIX, MN/CA IX, endogenous protein p54/58N

UniProt: Q16790