Recombinant MHC Class II antibody

Catalog No. ABIN7542975

The Rabbit Chimeric anti-MHC Class II antibody has been validated for FACS, WB, IHC, IP, IF, RIA and ICC. It is suitable to detect MHC Class II in samples from Rat.

Quick Overview for Recombinant MHC Class II antibody (ABIN7542975)

Target: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Antibody Type: Recombinant Antibody

Reactivity: Rat

Host: Rabbit

Clonality: Chimeric

Conjugate: This MHC Class II antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Radioimmunoassay (RIA), Immunocytochemistry (ICC)

Clone: OX-6

Product Details anti-MHC Class II Antibody

Purpose: Anti-MHC II [OX-6], Rabbit IgG, kappa

Specificity: This antibody recognises a monomorphic determinant of the rat MHC II (I-A) antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. It also cross-reacts with mouse MHC, specifically I-A[k] and I-A[s]

Cross-Reactivity: Mouse

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This antibody was prepared by subcutaneously injecting non-polymorphic MHC class II (Ia-like glycoproteins) from the membranes of rat thymocytes into BALB/c mice.

Isotype: IgG kappa

Application Details

Application Notes: This antibody binds to the rat MHC II (I-A) antigen, and cross-reacts with mouse I-A[k] and I-A[s]. Consequently, this antibody can be used to assess the tissue distribution of rat Ia antigens, indicating high Ia antigen expression on peripheral B lymphocytes, and significantly lower expression on thymocytes and T lymphocytes (McMaster, 1979).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for MHC Class II

Target: MHC Class II (MHC2) (Major Histocompatibility Complex Class II Antigen (MHC2))

Alternative Name: MHC II

Background: MHC Class II, MRC OX-6, CD74, MRC OX 6, Major Histocompatibility complex II