Recombinant CD86 antibody

Catalog No. ABIN7543020

The Mouse Monoclonal anti-CD86 antibody has been validated for FACS and IF. It is suitable to detect CD86 in samples from Rat.

Quick Overview for Recombinant CD86 antibody (ABIN7543020)

Target: CD86

Antibody Type: Recombinant Antibody

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD86 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: OX-48

Product Details anti-CD86 Antibody

Purpose: Anti-CD86 [OX-48], Mouse IgG1, kappa

Specificity: OX-48 binds specifically to CD86 targets which are present on subsets of T and B blasts, spleen macrophages and polymorphs, peritoneal macrophages, dendritic cells. OX48 does not appear to bind the same epitope of CD86 as 24F (Stephens et al. 2004). CD86 is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes - expression is rapidly upregulated by B cell specific stimuli. CD86 acts as a ligand for CD28 and CTLA-4. CD86 works in tandem with CD80 to prime T cells. 2 splice isoforms exist.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: OX-48 was prepared by immunizing mice with activated rat T cell blasts

Isotype: IgG1 kappa

Application Details

Application Notes: OX-48 blocks IL-2 production and proliferation of T cells (except when in exponential phase) (Samoza et al. 1990). The antibody can also be used for FC (Stephens et al. 2004), FACS and IPs. Ox-48 has been shown to be able to block binding of CD86 with its ligand CD28.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD86

Target: CD86

Alternative Name: CD86

Background: B72, B-72, OX48, MRC OX-48, MRC OX48

UniProt: Q62810

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation