Recombinant CD48 antibody

Catalog No. ABIN7543023

The Rabbit Chimeric anti-CD48 antibody has been validated for FACS and BP. It is suitable to detect CD48 in samples from Rat.

Quick Overview for Recombinant CD48 antibody (ABIN7543023)

Target: CD48

Antibody Type: Recombinant Antibody

Reactivity: Rat

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD48 antibody is un-conjugated

Application: Flow Cytometry (FACS), Blocking Peptide (BP)

Clone: OX-45

Product Details anti-CD48 Antibody

Purpose: Anti-CD48 [OX-45], Rabbit IgG, kappa

Specificity: OX-45 binds specifically to CD48 on rat thymocytes, T and B cells and endothelium but not LFA.3. Directly competes with CD46 for binding the target epitope. CD48 is a CD2 ligand and is expressed on the surface of all hematopoietic cells and endothelium. May be involved in the facilitation of interactions between activated lymphocytes. Likely to be involved in T cell activation.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: OX-45 was prepared by immunizing BALB/c mice with rat T blasts prepared by stimulating purified T helper (TH) cells with allogeneic irradiated rat spleen cells.

Isotype: IgG kappa

Application Details

Application Notes: Describes the use of OX-45 in blocking the primary mixed lymphocyte response (MLR)and the secondary response of T lymphocytes to the soluble antigen DNP-BGG. It also showed that OX-45 did not have any effect on the cytotoxic activity of specific (CTL) or non-specific (NK) killer cells or on proliferative responses stimulated by lectins or oxidative mitogenesis.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD48

Target: CD48

Alternative Name: CD48

Background: BCM1, BCM1 surface antigen, BLAST1, BLAST-1, HM48-1, MRC OX-45 surface antigen, MRC OX45 surface antigen, OX45 surface antigen, OX-45 surface antigen, SLAM family member 2, SLAMF2, sgp60, sgp-60, signalling lymphocytic activation molecule 2 OX45, MRC OX-4

UniProt: P10252