Recombinant CD43 antibody

Catalog No. ABIN7543048

This anti-CD43 antibody is a Rabbit Chimeric antibody detecting CD43 in FACS, WB and RIA. Suitable for Rat.

Quick Overview for Recombinant CD43 antibody (ABIN7543048)

Target: CD43 (SPN) (Sialophorin (SPN))

Antibody Type: Recombinant Antibody

Reactivity: Rat

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD43 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Radioimmunoassay (RIA)

Clone: OX-58

Product Details anti-CD43 Antibody

Purpose: Anti-CD43 [OX-58], Rabbit IgG, kappa

Specificity: This antibody recognises rat leukosialin, a major cell-surface sialoglycoprotein of thymocytes and T lymphocytes. It is also expressed on neutrophils, bone marrow stem cells and B-lineage cells after activation. The specificity of the leukosialin epitope is largely determined by linear protein sequence, as indicated by the reaction of this antibody with the extracellular domain of leukosialian expressed in an unglycosylated form in E. coli. However, the sensitivity of this antibody to neuraminidase treatment of leukosialin indicates that glycosylation can modify epitope conformation or flexibility.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogen: This antibody was prepared by immunizing BALB/c mice with rat thymocyte leukosialin and thymocytes.

Isotype: IgG kappa

Application Details

Application Notes: This antibody binds to rat leukosialin, and can be used to label thymocytes and T lymphocytes in the lymph node cell population in flow cytometry analysis. This antibody has also been used, along with other anti-CD43 MABs, in a Western blot analysis to localize leukosialin epitopes.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Alternative Name: CD43

Background: Leukosialin, Leukocyte sialoglycoprotein, Sialophorin, W3/13 antigen, OX 58, MRC OX 58

UniProt: P13838

Pathways: Regulation of Leukocyte Mediated Immunity