Recombinant SIRPG antibody

Catalog No. ABIN7543055

This Rabbit Chimeric antibody specifically detects SIRPG in ELISA, FACS, IP and BR. It exhibits reactivity toward Human.

Quick Overview for Recombinant SIRPG antibody (ABIN7543055)

Target: SIRPG (Signal-Regulatory Protein gamma (SIRPG))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This SIRPG antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Blocking Reagent (BR)

Clone: OX-119

Product Details anti-SIRPG Antibody

Purpose: Anti-SIRP g [OX-119], Rabbit IgG, kappa

Specificity: Hybridoma supernatants were initially screened by ELISA, using SIRPg-CD4, human SIRPa-Fc, human SIRPb-Fc or CD4 to eliminate hybridomas with cross-reactivity to rat CD4, human SIRPa, and SIRPb. There is some marginal binding of OX-119 to SIRPb transfected 293T cells. SIRPg binds CD47. On binding with CD47, mediates cell-cell adhesion. Engagement on T-cells by CD47 on antigen-presenting cells results in enhanced antigen-specific T-cell proliferation and costimulates T-cell activation. Expressed on CD4+ T-cells, CD8+ T-cells, CD56-bright natural killer (NK) cells, CD20+ cells, and all activated NK cells..

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: OX-119 was prepared by immunizing BALB/c mice with a chimeric protein made up of human SIRPg and a leader peptide of rat CD4 domain (SIRPg-CD4 ).

Isotype: IgG kappa

Application Details

Application Notes: OX-119 is able to perform staining of peripheral blood myeloid cells (PBMCs) and block SIRPg-mediated apoptosis. OX-119 can also be used in IP, FC and ELISA.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for SIRPG

Target: SIRPG (Signal-Regulatory Protein gamma (SIRPG))

Alternative Name: SIRP g

Background: CD172g, OX119, MRC OX-119, MRC OX119, SIRP-g, SIRPg, SIRP gamma, SIRP-gamma, CD172 antigen-like family member B, signal-regulatory protein beta, CD172-g

UniProt: Q9P1W8