Recombinant CD43 antibody

Catalog No. ABIN7543056

The Rabbit Chimeric anti-CD43 antibody has been validated for WB and IP. It is suitable to detect CD43 in samples from Rat.

Quick Overview for Recombinant CD43 antibody (ABIN7543056)

Target: CD43 (SPN) (Sialophorin (SPN))

Antibody Type: Recombinant Antibody

Reactivity: Rat

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD43 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: OX-56

Product Details anti-CD43 Antibody

Purpose: Anti-CD43 [OX-56], Rabbit IgG, kappa

Specificity: OX-56 binds specifically to CD43 and binding is not affected by CD43 glycosylation. OX-56 reacts with most thymocytes and T lymphocytes in the lymph node cell population. This antibody also binds to pre-B cells and can detect CD43 at low levels on B cells. CD43 plays a role in the physicochemical properties of the T-cell surface and in lectin binding. CD43 is a major cell-surface sialoglycoprotein - also found on neutrophils, bone marrow stem cells, B-lineage cells after activation of thymocytes and T lymphocytes but is not expressed on resting B lymphocytes. The protein presents carbohydrate ligands to selectins and Is a counter-receptor for SN/Siglec-1. During T-cell activation it is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogen: OX-56 was prepared by immunizing BALB/c mice with activated rat T lymphocytes.

Isotype: IgG kappa

Application Details

Application Notes: OX-56 has been used in the purification of rat leukosialin by immunoaffinty chromatography (Willis et al. 1987) as well as in WB.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD43

Target: CD43 (SPN) (Sialophorin (SPN))

Alternative Name: CD43

Background: Leukosialin, OX56, MRC OX-56, MRC OX56, sialophorin

UniProt: P13838

Pathways: Regulation of Leukocyte Mediated Immunity