Recombinant TNF alpha (Adalimumab Biosimilar) antibody

Catalog No. ABIN7543059

The Mouse Chimeric anti-TNF alpha (Adalimumab Biosimilar) antibody has been validated for ELISA, BR, IF and IHC. It is suitable to detect TNF alpha (Adalimumab Biosimilar) in samples from Human.

Quick Overview for Recombinant TNF alpha (Adalimumab Biosimilar) antibody (ABIN7543059)

Target: TNF alpha (Adalimumab Biosimilar)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Chimeric

Conjugate: This TNF alpha (Adalimumab Biosimilar) antibody is un-conjugated

Application: ELISA, Blocking Reagent (BR), Immunofluorescence (IF), Immunohistochemistry (IHC)

Clone: D2E7

Product Details anti-TNF alpha (Adalimumab Biosimilar) Antibody

Purpose: Anti-TNF alpha [D2E7 (Adalimumab)], Mouse IgG2a, kappa

Specificity: Binds to soluble TNF-alpha, but not to lymphotoxin (TNF beta).

Cross-Reactivity: Mouse

Characteristics:

Original Species of Ab: Human

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This antibody was generated by affinity maturation from the 2SD4 scFv, which in turn was selected on human TNF alpha by guided phage-display technology, using the murine anti-hTNF alpha antibody Mab32 as a template (c.f. also US Patent 6090382).

Isotype: IgG2a kappa

Application Details

Application Notes: Adalimumab binds specifically to TNF and neutralizes the biological function of TNF by blocking its interaction with the p55 and p75 cell surface TNF receptors. Adalimumab, unlike Infliximab (Ab00146), was shown to be cross-reactive with mouse TNF alpha (Arnoult 2017, PMID: 28584008).

Comment:

This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for TNF alpha (Adalimumab Biosimilar)

Target: TNF alpha (Adalimumab Biosimilar)

Target Type: Biosimilar

Background: Tumor necrosis factor alpha

UniProt: P01375