Recombinant EGFR (Nimotuzumab Biosimilar) antibody

Catalog No. ABIN7543062

The Mouse Chimeric anti-EGFR (Nimotuzumab Biosimilar) antibody has been validated for BR, FACS and IHC. It is suitable to detect EGFR (Nimotuzumab Biosimilar) in samples from Human.

Quick Overview for Recombinant EGFR (Nimotuzumab Biosimilar) antibody (ABIN7543062)

Target: EGFR (Nimotuzumab Biosimilar)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Chimeric

Conjugate: This EGFR (Nimotuzumab Biosimilar) antibody is un-conjugated

Application: Blocking Reagent (BR), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Clone: H-R3

Product Details anti-EGFR (Nimotuzumab Biosimilar) Antibody

Purpose: Anti-EGFR domain III [h-R3 (Nimotuzumab)], Mouse IgG2a, kappa

Specificity: Binds to human EGFR domain III at a site distinct from antibody 7A7 (Ab00134) (Talavera et al. 2011)

Characteristics:

Original Species of Ab: Human

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This antibody we generated by humanisation of the murine antibody R3, raised against EGFR purified from placenta membranes and A431 tumors.

Isotype: IgG2a kappa

Application Details

Application Notes: This antibody binds to human EGFR and inhibits EGF-dependent clonogenic growth of NRK cells and completely blocks EGF stimulated autophosphorylation of the receptor (Fernandez 1992)

Comment:

This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for EGFR (Nimotuzumab Biosimilar)

Target: EGFR (Nimotuzumab Biosimilar)

Target Type: Biosimilar

Background: ERBB-D3, ERBB1, HER1, PIG61, mENA, avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, proto-oncogene c-ErbB-1, receptor tyrosine-protein kinase erbB-1, epidermal growth factor

UniProt: P00533