Recombinant CD68 antibody

Catalog No. ABIN7543090

This anti-CD68 antibody is a Rabbit Chimeric antibody detecting CD68 in FACS, IHC and IP. Suitable for Mouse.

Quick Overview for Recombinant CD68 antibody (ABIN7543090)

Target: CD68 (CD68 Molecule (CD68))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD68 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Clone: FA-11

Product Details anti-CD68 Antibody

Purpose: Anti-CD68 [FA-11], Rabbit IgG, kappa

Specificity: This antibody is specific for murine CD68, known also as macrosialin (MS). CD68 is a 110 kDa, heavily O- and N- glycosylated type I integral membrane protein belonging to the sialomucin family and is closely related to the lysosomal/endosomal-associated membrane glycoproteins (LAMP). CD68 is predominantly expressed on the intracellular lysomsomes of monocytes and macrophages and to a lesser extent by dendritic cells and peripheral blood granulocytes. Elevated expression of CD68 has been demonstrated on CD34+ cells in various human malignancies, including several acute myeloid leukemia studies.

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunising rats (AO x Lou strain) with murine macrosialin (MS).

Isotype: IgG kappa

Application Details

Application Notes: When it was first generated, this antibody was used in binding assays, immunoprecipitation (IP) and immunofluorescence (IF) to study the differential expression of murine macrophage surface glycoprotein antigens in intracellular membranes (Smith et al., 1987). Subsequently, this antibody has been utilised in numerous FACS, IP, IHC, ICC/IF, WB applications, for instance, to delineate the roles of mouse and human CD68 as macrophage receptors for oxidised low density lipoprotein (Ramprasad et al., 1996), to indicate that mice unresponsive to granulocyte-macrophage colony-stimulating factor (GM-CSF) are unexpectedly resistant to cutaneous Leishmania major infection (Scott et al., 2000), to characterise the macrophage subsets harbouring Leishmania donovani in spleens of infected BALB/c mice (Lang et al., 2000), and to examine the role of Foxp3(+) Tregs in Alzheimer's disease (Baruch et al., 2015).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD68

Target: CD68 (CD68 Molecule (CD68))

Alternative Name: CD68

Background: Macrosialin, FA/11, FA11 antigen

UniProt: P31996