Recombinant PD-L1 antibody

Catalog No. ABIN7543101

The Rabbit Chimeric anti-PD-L1 antibody has been validated for FACS, IHC, ELISA and BP. It is suitable to detect PD-L1 in samples from Mouse.

Quick Overview for Recombinant PD-L1 antibody (ABIN7543101)

Target: PD-L1 (CD274 (PD-L1))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This PD-L1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Blocking Peptide (BP)

Clone: 10F-9G2

Product Details anti-PD-L1 Antibody

Purpose: Anti-PD-L1 [10F.9G2], Rabbit IgG, kappa

Specificity: This antibody is specific for murine PD-L1.

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunising Lewis rats with murine PD-L1 cDNA and murine PD-L1 CHO transfectants.

Isotype: IgG kappa

Application Details

Application Notes: The specificity of this antibody has been confirmed in ELISA analysis (Eppihimer et al, 2002). This antibody reacts specifically with mPD-L1-transfected cells in flow cytometry, and has been used to assess microvascular endothelial cells PD-L1 expression (Eppihimer et al, 2002). When administered to mice, a radiolabelled version of this antibody has been used to quantify PD-L1 expression in vivo, by measuring antibody accumulation in different tissues (Eppihimer et al, 2002). This antibody has also been used in immunohistochemical analysis of PD-L1 expression in frozen tissue sections of murine brain (Eppihimer et al, 2002) and murine hearts (Rodig et al, 2003). This antibody has been shown to block the binding of PD-L1 to both PD-1 and B7-1 (Paterson et al, 2011). This antibody precipitates diabetes in NOD mice and in adoptive transfer models of CD4+ and CD8+ T cell-driven diabetes, and accelerates diabetes in recipients of T cells from diabetic and prediabetic mice (Paterson et al, 2011).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for PD-L1

Target: PD-L1 (CD274 (PD-L1))

Alternative Name: PD-L1

Background: CD274, PDL1, PD L1, Programmed cell death 1 ligand 1, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1, 10F9G2, 10F 9G2

UniProt: Q9EP73

Pathways: Cancer Immune Checkpoints