Recombinant beta Catenin antibody

Catalog No. ABIN7543129

This anti-beta Catenin antibody is a Mouse Monoclonal antibody detecting beta Catenin in WB, IHC, FACS, IF and EMSA. Suitable for Human.

Quick Overview for Recombinant beta Catenin antibody (ABIN7543129)

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta Catenin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Electrophoretic Mobility-Shift Assay (EMSA)

Clone: 15B8

Product Details anti-beta Catenin Antibody

Purpose: Anti-beta catenin [15B8], Mouse IgG1, kappa

Specificity: This antibody recognizes beta catenin. It does not react with plakoglobin. β-Catenin plays a dual function in the cell including cell-cell adhesion and as a component of the Wnt (wingless) signaling pathway.

Cross-Reactivity: Chicken, Cow, Dog, Mouse, Rat

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunizing mice with a recombinant full length beta-catenin of chicken origin.

Isotype: IgG1 kappa

Application Details

Application Notes: This antibody is recommended for the detection and analysis of beta-catenin via various methods, such as Western blot, flow cytometry and immunofluorescence of cells and tissues. For instance, the antibody 15B8 was utilized in labelling beta-catenin in oral mucosa samples via immunohistochemistry (Das et al., 2013). Another group used this antibody in the identification of beta-catenin in extracts from K562 cells via Western blots (Kotula et al., 2014). Furthermore, this antibody was applied during immunohistochemistry stainings of transverse sections taken through the midbrain region of 8-9ss embryos (Schiffmacher et al., 2016). Finally,beta-catenin is a very popular marker used to outline the cell membrane, this antibody might be utilized for such purposes (Zhang et al., 2017).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for beta Catenin

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Alternative Name: beta catenin

Background: Catenin beta-1, Beta-catenin, β-catenin, β catenin

UniProt: P35222

Pathways: Peptide Hormone Metabolism