Recombinant beta Catenin antibody

Catalog No. ABIN7543130

This Rabbit Chimeric antibody specifically detects beta Catenin in WB, IHC, FACS, IF and EMSA. It exhibits reactivity toward Human.

Quick Overview for Recombinant beta Catenin antibody (ABIN7543130)

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This beta Catenin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Electrophoretic Mobility-Shift Assay (EMSA)

Clone: 15B8

Product Details anti-beta Catenin Antibody

Purpose: Anti-beta catenin [15B8], Rabbit IgG, kappa

Specificity: This antibody recognizes beta catenin. It does not react with plakoglobin. β-Catenin plays a dual function in the cell including cell-cell adhesion and as a component of the Wnt (wingless) signaling pathway.

Cross-Reactivity: Chicken, Cow, Dog, Mouse, Rat

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunizing mice with a recombinant full length beta-catenin of chicken origin.

Isotype: IgG kappa

Application Details

Application Notes: This antibody is recommended for the detection and analysis of beta-catenin via various methods, such as Western blot, flow cytometry and immunofluorescence of cells and tissues. For instance, the antibody 15B8 was utilized in labelling beta-catenin in oral mucosa samples via immunohistochemistry (Das et al., 2013). Another group used this antibody in the identification of beta-catenin in extracts from K562 cells via Western blots (Kotula et al., 2014). Furthermore, this antibody was applied during immunohistochemistry stainings of transverse sections taken through the midbrain region of 8-9ss embryos (Schiffmacher et al., 2016). Finally,beta-catenin is a very popular marker used to outline the cell membrane, this antibody might be utilized for such purposes (Zhang et al., 2017).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for beta Catenin

Target: beta Catenin (CATNB) (Catenin, beta (CATNB))

Alternative Name: beta catenin

Background: Catenin beta-1, Beta-catenin, β-catenin, β catenin

UniProt: P35222

Pathways: Peptide Hormone Metabolism