Recombinant Fc gamma RII (CD32) antibody

Catalog No. ABIN7543151

This Mouse Monoclonal antibody specifically detects in FACS, ELISA and BR. It exhibits reactivity toward Human.

Quick Overview for Recombinant Fc gamma RII (CD32) antibody (ABIN7543151)

Target: Fc gamma RII (CD32)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), ELISA, Blocking Reagent (BR)

Clone: MDE-8

Product Details

Purpose: Anti-CD32 [MDE-8], Mouse IgG1, kappa

Specificity: This antibody recognizes and binds Fc-gamma receptor IIa (FcγRIIa) or CD32a on peripheral blood monocytes, polymorphonuclear cells and platelets.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing Human Ig-Tg (Co7) mice with FcgammaRIIa-H131 conjugated to human serum albumin in complete Freund's adjuvant.

Isotype: IgG1 kappa

Application Details

Application Notes: The binding ability of MDE-8 to FcgammaRIIa was confirmed using flow cytometry. MDE-8 binds to FcgammaRIIa on cell lines, peripheral bloodmonocytes, PMN, platelets and blocked ligand binding via this receptor. It can also effectively blocked FcγRIIa ligand-binding via its F(ab')2 fragment. Overnight incubation of monocytes with F(ab')2 fragments of MDE-8 leads to a 60 % decrease in cell surface expression of FcγRIIa. MDE-8 whole antibody induced a concomitant 30 % decrease of FcγRI on THP-1cells and monocytes (PMID: 15982355). MDE-8 was reoported to induce Fc-mediated hypothermia and severe thrombocytopenia in FCGR2A mice (PMID: 26396093).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details

Target: Fc gamma RII (CD32)

Alternative Name: CD32

Background: CDw32, Fc-gamma RII-a, FcγRIIa, FcγRII, FcRII-a, FCGR2A, IgG Fc receptor II-a, Low affinity immunoglobulin gamma Fc region receptor II-a

UniProt: P12318