Recombinant Fc gamma RII (CD32) antibody

Catalog No. ABIN7543154

This Rabbit Chimeric antibody specifically detects in FACS, IHC, ELISA, IF and BR. It exhibits reactivity toward Human.

Quick Overview for Recombinant Fc gamma RII (CD32) antibody (ABIN7543154)

Target: Fc gamma RII (CD32)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Blocking Reagent (BR)

Clone: MDE-9

Product Details

Purpose: Anti-CD32 [MDE-9], Rabbit IgG, kappa

Specificity: This antibody recognizes and binds Fc-gamma receptor IIa (FcγRIIa) or CD32a and, more particularly, the FcγRIIa-H131 allotype.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing Human Ig-Tg (Co7) mice with FcgammaRIIa-H131 conjugated to human serum albumin in complete Freund's adjuvant.

Isotype: IgG kappa

Application Details

Application Notes: MDE-9 was able to detect FcgammaRIIa-H131 in both flow cytometry and immunohistochemistry. MDE-9 bound FcgRIIa-H131 on cell lines expressing FcgRIIaH131, as well as on peripheral blood monocytic cells and PMN in immunofluorescence assays. MDE-9 was used to develop an FcgammaRIIa allotyping method based on flow cytometry. The results of flow cytometric FcgammaRIIa allotyping correlated completely with PCR genotyping. This novel allotyping assay can facilitate the screening of patients in a routine diagnostic setting. A combination of MDE-9 and mAb 41H16 can be used in FcgammaRIIa-H/H131 homozygous individuals to detect FcgammaRIIa and FcgammaRIIb surface expression on monocytes (PMID: 15604023).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details

Target: Fc gamma RII (CD32)

Alternative Name: CD32

Background: CDw32, Fc-gamma RII-a, FcγRIIa, FcγRII, FcRII-a, FCGR2A, FcgammaRIIa-H131, IgG Fc receptor II-a, Low affinity immunoglobulin gamma Fc region receptor II-a

UniProt: P12318