Recombinant CEACAM8 antibody

Catalog No. ABIN7543167

The Rabbit Chimeric anti-CEACAM8 antibody has been validated for FACS, IF, ISG and ISt. It is suitable to detect CEACAM8 in samples from Human.

Quick Overview for Recombinant CEACAM8 antibody (ABIN7543167)

Target: CEACAM8 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 8 (CEACAM8))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This CEACAM8 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunoscintigraphy (ISG), Immunostaining (ISt)

Clone: BW 250-183

Product Details anti-CEACAM8 Antibody

Purpose: Anti-CD66b [BW 250/183], Rabbit IgG, kappa

Specificity: This antibody binds the human CD66b, which is expressed on the surface of granulocytes.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing mice with CEA positive adenocarcinoma cells preferably from lungs.

Isotype: IgG kappa

Application Details

Application Notes: This murine antibody reacted with granulocytes, colon mucosa and faintly with pancreatic ducts (PMID: 2410375). The radioactive isotope technetium-99m labelled version of this antibody is also called Besilesomab (trade name Scintimun). It has been studied widely for therapeutic applications. Technetium-99m-Labeled BW 250/183 was used to study the inflamatory process and identification of the inflamatory foci using immunoscintigraphy (PMID: 2157831, 1565572). This antibody is also used for scintiographic imaging of soft tissue infections, peripheral bone, and abdominal infections (PMID: 8023170). Tc-99m-labeled antibody has also been used in the localization of extramedullary hematopoiesis (PMID: 11953571). Rhenium-188-labelled BW250/183 was developed for adjuvant conditioning radioimmunotherapy of bone marrow before bone marrow transplantation. Immunoreactivity of the reduced and rhenium-incorporated antibody was determined by indirect immunofluorescence, by means of a whole blood staining method follwoed by flow ctometric analysis (PMID: 10541824). 90Y-label is reportedled antibody is reported to be used in the study of expression of CD66b in non-Hodgkin lymphomas and multiple myeloma usinf flow cytometry (PMID: 20887387).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CEACAM8

Target: CEACAM8 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 8 (CEACAM8))

Alternative Name: CD66b

Background: CEACAM8, CGM6, Carcinoembryonic antigen-related cell adhesion molecule 8, Carcinoembryonic antigen CGM6, CD67 antigen, Non-specific cross-reacting antigen, NCA-95

UniProt: P31997