Recombinant SARS-CoV-2 Nucleocapsid antibody

Catalog No. ABIN7543169

This Rabbit Chimeric antibody specifically detects SARS-CoV-2 Nucleocapsid in ELISA, WB, FACS and IHC. It exhibits reactivity toward SARS Coronavirus-2 (SARS-CoV-2).

Quick Overview for Recombinant SARS-CoV-2 Nucleocapsid antibody (ABIN7543169)

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Antibody Type: Recombinant Antibody

Reactivity: SARS Coronavirus-2 (SARS-CoV-2)

Host: Rabbit

Clonality: Chimeric

Conjugate: This SARS-CoV-2 Nucleocapsid antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC)

Clone: 1C7C7

Product Details anti-SARS-CoV-2 Nucleocapsid Antibody

Purpose: Anti-Covid-19 & SARS-CoV Nucleocapsid [1C7C7], Rabbit IgG, kappa

Specificity: This antibody specifically targets an epitope on the SARS-CoV-2 and SARS-CoV nucleocapsid protein and does not cross-react with the nucleocapsid of other common human coronaviruses, OC43 and 229E.

Cross-Reactivity: SARS Coronavirus (SARS-CoV)

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: The original version of this antibody was raised against SARS-CoV-1/2.

Isotype: IgG kappa

Application Details

Application Notes: The antibody 1C7C7 detects SARS-CoV-2 and SARS-CoV-1 and can be used in affinity binding assays, flow cytometry, immunocytochemistry, and western blotting. Its effectiveness in detecting the nucleocapsid of the novel coronavirus causing COVID-19 was confirmed in multiple studies. For instance, it was used to analyze SARS-CoV-2 nucleoprotein extracted from infected cells via Western blot (Hoagland et al., 2020) ,and similarly by Blanco-Melo et al. in a study on imbalanced host response to SARS-CoV-2 (2020, pmid: 32416070). Another group has used this clone to stain infected cells fixed with 10 % neutral formalin during a neutralization assay (Piepenbrink et al., 2020). Daniloski et al. performed similar staining procedure with 1C7C7 for a trans-complementation assay (2020, pmid: 33147445). Finally, this antibody was also used to stain SARs-CoV-2 infected cells in a study on in vitro susceptibility to SARS-CoV-2 infection (Dobrindt et al., 2020, pmid: 32995783).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for SARS-CoV-2 Nucleocapsid

Target: SARS-CoV-2 Nucleocapsid (SARS-CoV-2 N)

Alternative Name: SARS-CoV-2 Nucleocapsid

Target Type: Viral Protein

Background: Nucleoprotein, NP, NC, Protein N, Nucleocapsid protein, SARS-CoV Protein N, SARS-CoV-2 Nucleocapsid protein, SARS Coronavirus, SARS-CoV-2, SARS CoV 2, 2019-nCoV, 1C7, severe acute respiratory syndrome virus

UniProt: P0DTC9