Recombinant CD1d antibody

Catalog No. ABIN7543209

The Rabbit Chimeric anti-CD1d antibody has been validated for FACS, IHC, ELISA and IF. It is suitable to detect CD1d in samples from Mouse.

Quick Overview for Recombinant CD1d antibody (ABIN7543209)

Target: CD1d (CD1D) (CD1d Molecule (CD1D))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD1d antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Clone: 1B1

Product Details anti-CD1d Antibody

Purpose: Anti-CD1d [1B1], Rabbit IgG, kappa

Specificity: This antibody is specific for CD1d. Its binding is blocked by antibody clone 3D11. CD1d is an antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells.

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogen: The original version of this antibody was raised by immunizing Lewis rats using mCD1-transfected RMA-S cells.

Isotype: IgG kappa

Application Details

Application Notes: In an attempt to further the understanding of CD1 proteins, flow cytometry was preformed on mouse spleen cells using the rat version of this antibody. Further, mice infected with L. monocytogenes were treated with the rat version of this antibody. Finally, mice spleen cells were stained with the mouse version of this antibody (Szalay et al, 1999, pmid:10358132). To investigate the tissue distribution of murine CD1 in mice, immunohistochemistry was preformed on thymus, small intestine, and spleen tissue of mice using the rat version of this antibody. Further, flow cytometry was preformed on epithelial cells and IEL using the rat version of this antibody (Brassay et al, 1997, pmid:9233616). In order to measure mCD1-restricted T cell responses to glycolipids, the rat version of this antibody was added after which cytokine levels were assessed by ELISA (Burdin et al, 1998, pmid:9759842). To assess the role of CD1 in the pathogenesis of lupus the rat version of this antibody was used for immunofluorescent staining and flow cytometry on single cell suspensions of mouse spleen cells or mouse bone marrow. The mouse version of this antibody was further tested in vivo on mice with lupus. Their lifespan showed an increase (Zeng et al, 2000, pmid:10799851). To study the expression of CD1 on B cell subsets from normal and b2m2/2 mice, single-cell suspensions of lymphoid cells from C57BL/6 and BALB/c mice were used for immunofluorescence using the mouse version of this antibody (Amano et al, 1998, pmid:9712035). To eludicate the location of expression of CD1 flow cytometry was preformed on thymocytes from wild-type, b2m-deficient, and TAP-1-deficient C57BL/6 mice (Roark et al, 1998, pmid:9531266).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD1d

Target: CD1d (CD1D) (CD1d Molecule (CD1D))

Alternative Name: CD1d

Background: Antigen-presenting glycoprotein CD1d1, Cd1d1, CD1d.1

UniProt: P11609

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process