Recombinant FSHR antibody

Catalog No. ABIN7543214

This Rabbit Chimeric antibody specifically detects FSHR in ELISA, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for Recombinant FSHR antibody (ABIN7543214)

Target: FSHR (Follicle Stimulating Hormone Receptor (FSHR))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This FSHR antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 9H11

Product Details anti-FSHR Antibody

Purpose: Anti-FSH receptor [9H11], Rabbit IgG, lambda

Specificity: This antibody binds human and mice FSH receptor. FSH receptor is a G protein-coupled receptor for follitropin, the follicle-stimulating hormone. Through cAMP production activates the downstream PI3K-AKT and ERK1/ERK2 signaling pathways.

Cross-Reactivity: Mouse

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing Balb/c mice with an optimized DNA construct encoding human FSHR.

Isotype: IgG lambda

Application Details

Application Notes: To test if the antibody dependent cellular cytotoxicity elicited by the mouse version of this antibody translated into an in vivo anti-tumor effect, mice were challenged with IDS -Defl29/Vegf-a-Fshr and treated with the mouse version of this antibody. The antibody was able to delay tumor progression. To further characterize the antibody its ability to bind cells expressing human and mouse FHSR, flow cytometry was performed using the mouse version of this antibody on A20 cells, ID8- Defl29/Vegf-a-Fshr cells and human ovarian cancer cell lines like Caov3, OVCAR3, TOV21 G and PC3 with physiological levels of FSHR. Isotype analysis of the antibody was done using ELISA. Finally immunofluorescence was performed on 293T cells transduced with human or murine FSHR using the mouse version of this antibody (WO2020033797).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for FSHR

Target: FSHR (Follicle Stimulating Hormone Receptor (FSHR))

Alternative Name: FSH receptor

Background: FSHR, FSH-R, Follicle-stimulating hormone receptor, Follitropin receptor, LGR1

UniProt: P23945

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Platelet-derived growth Factor Receptor Signaling