Recombinant FAP antibody

Catalog No. ABIN7543215

The Mouse Monoclonal anti-FAP antibody has been validated for IHC, ELISA and FACS. It is suitable to detect FAP in samples from Human.

Quick Overview for Recombinant FAP antibody (ABIN7543215)

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FAP antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Clone: 3F2

Product Details anti-FAP Antibody

Purpose: Anti-FAP [3F2], Mouse IgG2a, kappa

Specificity: This antibody can bind human, mouse and cynomolgus monkey fibroblast activation protein.

Cross-Reactivity: Cynomolgus, Mouse

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Isotype: IgG2a kappa

Application Details

Application Notes: The original mouse antibody was used to check the expression levels of FAP on the surface of GM05389 lung fibroblasts using flow cytometry. The original mouse IgG1 version of this antibody binds human, mouse and cynomolgus monkey FAP with an avidity of 39 pM, 29 pM and 42 pM respectively. Binding of human IgG1 antibody 3F2 to human and murine FAP expressed on stably transfected HEK293 cells, human fibroblasts cell line GM05389 was measured by floy cytometry. The mouse IgG2a version of this antibody was also used for the immunohistochemical stainings of frozen tissue microarray (TMA) containing 10 invasive ductal carcinomas of the breast, 10 colorectal adenocarcinomas and 10 non-small cell lung cancers (US20200079873).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for FAP

Target: FAP (Fibroblast Activation Protein, alpha (FAP))

Alternative Name: FAP

Background: Seprase, Fibroblast activation protein, Prolyl endopeptidase FAP, 170 kDa melanoma membrane-bound gelatinase, Dipeptidyl peptidase FAP, FAPalpha, Fibroblast activation protein alpha, Gelatin degradation protease FAP, Integral membrane serine protease, Post-proline cleaving enzyme, Serine integral membrane protease, SIMP, Surface-expressed protease

UniProt: Q12884

Pathways: Tube Formation