Recombinant PD-1 antibody

Catalog No. ABIN7543217

This Rabbit Chimeric antibody specifically detects PD-1 in FACS, WB, IHC and BP. It exhibits reactivity toward Mouse.

Quick Overview for Recombinant PD-1 antibody (ABIN7543217)

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Antibody Type: Recombinant Antibody

Reactivity: Mouse

Host: Rabbit

Clonality: Chimeric

Conjugate: This PD-1 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Blocking Peptide (BP)

Clone: 29F-1A12

Product Details anti-PD-1 Antibody

Purpose: Anti-PD-1 [29F.1A12], Rabbit IgG, kappa

Specificity: This antibody recognizes an epitope on mouse PD-1.

Characteristics:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: The antibody was raised by immunizing rats with plasmid DNA containing PD-1.

Isotype: IgG kappa

Application Details

Application Notes: This antibody was used for detection of PD-1 in CHO cells using flow cytometry. The ability of the antibody to block PD-1 interaction with PD-L1 or PD-L2 on CHO cells in an in vitro blocking assay was tested, the clone blocked PD-L1 Ig and PD-L2 Ig binding at high concentration, but only blocked 35 % of PD-L1 Ig or PD-L2 Ig binding at 10 μg/mL. This antibody was also used for immunohistochemical studies of various mouse tissues, such as heart (Liang et al, 2003, pmid: 14515254). In vivo anti-tumor effect in terms of level of tumor growth reduction was studied, a significant tumor growth reduction was observed in the tumors from mice treated with the antibody (Tobias et al, 2020, pmid: 32528470). The antibody was used for the detection of PD-1 in EL4 cells using flow cytometry. The antibody was used for cross-blocking with the unconjugated version of the same clones and it was reported to prevent PD-1 from interacting with PD-L1 (Polesso et al, 2021, pmid: 33684223). The antibody was used as a positive control for CT26 tumor model, a murine model of colon cancer (Kaumaya ey al., pmid:33117602).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for PD-1

Target: PD-1 (PDCD1) (Programmed Cell Death 1 (PDCD1))

Alternative Name: PD-1

Background: CD279, Programmed cell death protein 1, Protein PD-1, mPD-1

UniProt: Q02242

Pathways: Cancer Immune Checkpoints