Recombinant Fibrin beta-Chain (N-Term) antibody

Catalog No. ABIN7543222

Product Details

Target: Fibrin beta-Chain

Antibody Type: Recombinant Antibody

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: Un-conjugated

Application: ELISA

Purpose: Anti-Fibrin beta-chain [59D8], Rabbit IgG, kappa

Specificity: This antibody selectively binds to the amino-terminus of the fibrin β-chain that becomes exposed after cleavage of fibrinopeptide B by thrombin.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by using a synthetic heptapeptide from the amino terminus of the beta chain in human fibrin as an immunogen.

Clone: 59D8

Isotype: IgG kappa

Application Details

Application Notes: The original mouse antibody was able to bind fibrin even in the presence of human fibrinogen at the concentration found in plasma. This antibody when intravenously administered in a chicken ex vivo circulatory model bound human fibrin-coated disks placed in an extracorporeal chamber (PMID: 6648524). An immunoconjugate (tPA-59D8) generated from tissue plasminogen activator (tPA) and anti-fibrin antibody 59D8 degraded less fibrinogen and consumed less alpha 2-antiplasmin than did tPA alone, suggesting that more potent and more selective thrombolysis can be achieved by efficiently directing tPA to the site of a thrombus (PMID: 3118374). This antibody was used in the generation of a single-chain urokinase plasminogen activator (scu-PA) and -59D8 Fab' conjugate which had a fibrinolytic potency 33-fold more than that of tissue plasminogen activator (PMID: 2111744). A recombinant r-scuPA(32 kDa)-59D8 was 6 times more potent than high molecular mass scuPA in lysing a human plasma clot in vitro and was 20 times more potent than high molecular mass scuPA in the rabbit jugular vein model of thrombolysis. The binding affinity of the antibody towards fibrin was determined using ELISA (PMID: 1946453). This antibody was also used in the generation of a fusion protein comprising hirudin that genetically fused to the C-terminus of the variable region of the light chain of scFv59D8. This fusion protein was found to inhibit thrombin after its activation by factor Xa and also inhibits appositional clot growth in vitro (PMID: 10715263).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details

Target: Fibrin beta-Chain

Background: FGB, factor Ia, factor-1a, fibrin, fibrin β chain, fibrin beta chain, Fibrinogen beta chain, Fibrinopeptide B, Fibrinogen beta chain

UniProt: P02675