Recombinant CD2 antibody

Catalog No. ABIN7543282

This anti-CD2 antibody is a Rabbit Chimeric antibody detecting CD2 in FACS and IP. Suitable for Human.

Quick Overview for Recombinant CD2 antibody (ABIN7543282)

Target: CD2

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD2 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: 35-1

Product Details anti-CD2 Antibody

Purpose: Anti-CD2 [35.1], Rabbit IgG, kappa

Specificity: This antibody recognizes an epitope on the extracellular domain of the human T cell surface protein Tp50 (CD2) between amino acid residues 39-50. The binding of this antibody does not inhibit the interaction between CD2 and CD58. CD2 is a glycoprotein belonging to the immunoglobulin superfamily. It acts as a specific marker for T lymphocytes, natural killer cells, thymic B cell subsets, and some antigen presenting cells. CD2 interacts with CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing mice with human T lymphocytes that had been activated with phorbol myristic acid (PMA).

Isotype: IgG kappa

Application Details

Application Notes: This antibody inhibits T cell proliferative responses and cytotoxicity, but does not inhibit antibody mediated cellular cytotoxicity. This antibody was used in the generation of CD2 targeting ricin A chain-containing immunotoxins (PMID: 2461993). This antibody is capable of immunoprecipitating CD2 antigen form cell lysates (PMID: 8557753). This antibody was also used for the cell cycle analysis of cells along with other antibodies like anti-CD3, anti-CD4, and anti-CD8 using flow cytometry (PMID: 9881968).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD2

Target: CD2

Alternative Name: CD2

Background: LFA-2, LFA-3 receptor, SRBC, Rosette receptor, T-cell surface antigen CD2, Erythrocyte receptor, T-cell surface antigen T11/Leu-5

UniProt: P06729