Recombinant PSMA antibody

Catalog No. ABIN7543287

The Mouse Chimeric anti-PSMA antibody has been validated for WB, IHC, ELISA, FACS, IF and IP. It is suitable to detect PSMA in samples from Human.

Quick Overview for Recombinant PSMA antibody (ABIN7543287)

Target: PSMA (FOLH1) (Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Chimeric

Conjugate: This PSMA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: J591

Product Details anti-PSMA Antibody

Purpose: Anti-PSMA [J591], Mouse IgG1, lambda

Specificity: This antibody binds the extracellular domain of the human PSMA. Prostate-specific membrane antigen (PSMA) is a type II integral membrane glycoprotein belonging to the M28 peptidase family. It acts as a glutamate carboxypeptidase on different alternative substrate. It is highly expressed in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c mice with LNCaP cells or a primary culture of PCa epithelial cells.

Isotype: IgG1 lambda

Application Details

Application Notes: This antibody was used in the immunohistochemical analysis of PSMA expressed on renal, urothelial, colon, lung, breast carcinomas and metastatic adenocarcinoma of liver. This antibody can also immunoprecipitate PSMA from crude LNCaP lysates (PMID: 9288760). This antibody was also used for the identification of PSMA in a western blot assay. The epitope analysis of this antibody was done using ELISA. This antibody was also used in the immunofluorescence staining of on nonpermeabilized and permeabilized LNCaP cells (PMID: 9288760). This antibody was used in a flow cytometric study for enumeration of circulating prostate microparticles (PMPs), a type of extracellular vesicle (EV) released by prostate cancer present in seminal fluid, urine, and blood may represent a non-invasive means to identify and prioritize patients with intermediate and high risk of prostate cancer (PMID: 26814433). In a phase 1 trial in patients with advanced solid tumor malignancies this antibody showed acceptable toxicity and excellent targeting of known sites of metastases (PMID: 17290063).

Comment:

This chimeric mouse antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for PSMA

Target: PSMA (FOLH1) (Folate Hydrolase (Prostate-Specific Membrane Antigen) 1 (FOLH1))

Alternative Name: PSMA

Background: PSM, FOLH1, FOLH, FGCP, GCPII, mGCP, NAALAD1, GIG27, Prostate specific membrane antigen, Glutamate carboxypeptidase 2, Cell growth-inhibiting gene 27 protein, Folate hydrolase 1, Folylpoly-gamma-glutamate carboxypeptidase, Glutamate carboxypeptidase II, Membrane glutamate carboxypeptidase, N-acetylated-alpha-linked acidic dipeptidase I, NAALADase I, Prostate-specific membrane antigen, Pteroylpoly-gamma-glutamate carboxypeptidase

UniProt: Q04609