Recombinant CD3 epsilon antibody (AA 23-126)

Catalog No. ABIN7543303

This anti-CD3 epsilon antibody is a Mouse Monoclonal antibody detecting CD3 epsilon in WB, IHC and ELISA. Suitable for Human.

Quick Overview for Recombinant CD3 epsilon antibody (AA 23-126) (ABIN7543303)

Target: CD3 epsilon (CD3E)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 33H1

Product Details anti-CD3 epsilon Antibody

Binding Specificity: AA 23-126

Purpose: Anti-CD3e [33H1], Mouse IgG2a, kappa

Specificity: This antibody binds human CD3e. It is part of TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. It also plays a role in signal transduction during T cell development and in correct T-cell development.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c mice with recombinant human CD3 antigen (amino acids 23-126).

Isotype: IgG2a kappa

Application Details

Application Notes: This binding specificity of this antibody for human CD3e was checked using ELISA. The affinity constant of the original IgG2a antibody was determined to be 1.92x10^9 M^(-1). This antibody can recognize human CD3e recombinant protein in an immunoblot assay. This antibody is similar to commercially available anti-CD3 antibody SP7 in immunohistochemical staining of T cell lymphomas. This antibody can also be used for the immunohistochemical staining of normal tissues like brain, heart, cerebellum, esophageal, adrenal, stomach, ovary, small intestine, pancreatic, colorectal, paratony gland, liver, pituitary, salivary gland, testicular, kidney, thyroid, prostate, breast, uterus, spleen , Bladder, tonsil, skeletal muscle, thymus (young children), skin, bone marrow, peripheral nerve, lung and melodder cells (CN111363043).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3e

Background: T3E, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3-ε, CGMCC NO 21980, SP7

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway