Recombinant CD3 antibody

Catalog No. ABIN7543307

This Human Monoclonal antibody specifically detects CD3 in FACS and in vitro. It exhibits reactivity toward Human.

Quick Overview for Recombinant CD3 antibody (ABIN7543307)

Target: CD3

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Human

Clonality: Monoclonal

Conjugate: This CD3 antibody is un-conjugated

Application: Flow Cytometry (FACS), In vitro Assay (in vitro)

Clone: CD3B219

Product Details anti-CD3 Antibody

Purpose: Anti-CD3 [CD3B219], Human IgG4, lambda

Specificity: The antibody is specific for CD3 epsilon.

Cross-Reactivity: Monkey

Characteristics:

Original Species of Ab: Human

Original Format of Ab: IgG4

Purification: Protein A affinity purified

Isotype: IgG4 lambda

Application Details

Application Notes: The antibody was used to construct different CD33 xCD3 bispecific antibodies (IgG4). In vitro T cell mediated cytotoxicity assays were performed using the bispecific antibody and the cytotoxicity was measured by flow cytometry. Ex vivo cytotoxicity assay showed the paired antibody promoted a dose-dependent reduction of total cytotoxicity that correlated with T cell activation after 48 hours (US20210040209A1). The antibody is considered a high affinity binder (Kd= 8 mM) (US20210214440A1). The CD33xCD3 bispecific antibodies specifically induce cell cytotoxicity of MOLM-13 cells and CD33+ monocytes at 48 hr (US20200048349A1).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD3

Target: CD3

Alternative Name: CD3

Background: T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, SP34-2

UniProt: P07766

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway