Recombinant CD3 antibody

Catalog No. ABIN7543308

The Rabbit Chimeric anti-CD3 antibody has been validated for FACS and in vitro. It is suitable to detect CD3 in samples from Human.

Quick Overview for Recombinant CD3 antibody (ABIN7543308)

Target: CD3

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD3 antibody is un-conjugated

Application: Flow Cytometry (FACS), In vitro Assay (in vitro)

Clone: CD3B219

Product Details anti-CD3 Antibody

Purpose: Anti-CD3 [CD3B219], Rabbit IgG, lambda

Specificity: The antibody is specific for CD3 epsilon.

Cross-Reactivity: Monkey

Characteristics:

Original Species of Ab: Human

Original Format of Ab: IgG4

Purification: Protein A affinity purified

Isotype: IgG lambda

Application Details

Application Notes: The antibody was used to construct different CD33 xCD3 bispecific antibodies (IgG4). In vitro T cell mediated cytotoxicity assays were performed using the bispecific antibody and the cytotoxicity was measured by flow cytometry. Ex vivo cytotoxicity assay showed the paired antibody promoted a dose-dependent reduction of total cytotoxicity that correlated with T cell activation after 48 hours (US20210040209A1). The antibody is considered a high affinity binder (Kd= 8 mM) (US20210214440A1). The CD33xCD3 bispecific antibodies specifically induce cell cytotoxicity of MOLM-13 cells and CD33+ monocytes at 48 hr (US20200048349A1).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG4 format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD3

Target: CD3

Alternative Name: CD3

Background: T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, SP34-2

UniProt: P07766

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway