Recombinant Cytokeratin 18 antibody (Extracellular Domain)

Catalog No. ABIN7543320

The Rabbit Chimeric anti-Cytokeratin 18 antibody has been validated for ELISA. It is suitable to detect Cytokeratin 18 in samples from Human.

Quick Overview for Recombinant Cytokeratin 18 antibody (Extracellular Domain) (ABIN7543320)

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This Cytokeratin 18 antibody is un-conjugated

Application: ELISA

Clone: HH156

Product Details anti-Cytokeratin 18 Antibody

Binding Specificity: Extracellular Domain

Purpose: Anti-Cytokeratin 18 [HH156], Rabbit IgG, kappa

Specificity: This antibody is specific for extracellular domain of the human cytokeratin 18 containing amino acids 'LEDGEDFNLGDALD'. Cytokeratins are intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. Cytokeratin 18 is widely expressed in human monolayer epithelial tissues, and generally appears in pairs with CK8. CK18 is a serum marker for a variety of tumor diagnosis, as well as a serum marker for liver injury. It is involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, it plays a role in filament reorganization. It is also involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

Characteristics:

Original Species of Ab: Human

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c mice with human cytokeratin 18 extracellular domain polypeptide 'LEDGEDFNLGDALD'. Later on the humanized version of this antibody was generated by grafting the CDRs of the original mouse antibody E156 onto human framework regions.

Isotype: IgG kappa

Application Details

Application Notes: This antibody can bind human cytokeratin 18 in an ELISA. The original IgG1 version of this antibody is reported to bind human cytokeratin 18 with a binding affinity of 32.8 pM.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Alternative Name: Cytokeratin 18

Background: CK18, CK-18, CK 18, KRT18, CYK18, Keratin type I cytoskeletal 18, Cytokeratin-18, Cell proliferation-inducing gene 46 protein

UniProt: P05783

Pathways: Apoptosis, Caspase Cascade in Apoptosis