Recombinant KRT20 antibody (AA 411-424)

Catalog No. ABIN7543324

This anti-KRT20 antibody is a Rabbit Chimeric antibody detecting KRT20 in WB, IHC and ELISA. Suitable for Human.

Quick Overview for Recombinant KRT20 antibody (AA 411-424) (ABIN7543324)

Target: KRT20 (Keratin 20 (KRT20))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This KRT20 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 10F6B12A9

Product Details anti-KRT20 Antibody

Binding Specificity: AA 411-424

Purpose: Anti-Cytokeratin 20 [10F6B12A9], Rabbit IgG, kappa

Specificity: This antibody is specific for amino acids 411-424 of human cytokeratin 20. Cytokeratins are intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. There are 19 keratin polypeptides are found in human epithelium. Cytokeratin 20 plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, it plays a role in the secretion of mucin in the small intestine.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing female ICR mice with human CK20 peptide (amino acids 411-424) conjugated to KLH in equal volume of Freund's incomplete adjuvant.

Isotype: IgG kappa

Application Details

Application Notes: This antibody can bind human cytokeratin 20 in an ELISA. It is also capable of detecting human cytokeratin 20 in a western blot assay. This antibody is similar to commercially available anti-Cytokeratin 20 antibody KS20.8 in staining various human tissues. This antibody was used in the immunohistochemical staining of paraffin sections of stomach, colon and colonic cancer tissues (CN109734805).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for KRT20

Target: KRT20 (Keratin 20 (KRT20))

Alternative Name: Cytokeratin 20

Background: CK20, CK-20, K20, KRT20, Cytokeratin-20, Keratin-20, Keratin type I cytoskeletal 20 ,Protein IT, CGMCC No 16204, Ks20.8

UniProt: P35900