Recombinant KRT20 antibody

Catalog No. ABIN7543325

This Mouse Monoclonal antibody specifically detects KRT20 in WB, IHC and ELISA. It exhibits reactivity toward Human.

Quick Overview for Recombinant KRT20 antibody (ABIN7543325)

Target: KRT20 (Keratin 20 (KRT20))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This KRT20 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 10A2

Product Details anti-KRT20 Antibody

Purpose: Anti-Cytokeratin 20 [10A2], Mouse IgG2a, kappa

Specificity: This antibody binds human cytokeratin 20. Cytokeratins are intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. There are 19 keratin polypeptides are found in human epithelium. Cytokeratin 20 plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, it plays a role in the secretion of mucin in the small intestine.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing female ICR mice with recombinant human CK20 histidine-tagged fusion protein in Freund's complete adjuvant.

Isotype: IgG2a kappa

Application Details

Application Notes: The binding characterization of this antibody was done using ELISA. The affinity constant of the original IgG2a antibody was determined to be 1.92 x 10^9 M^(-1). This antibody was capable of detecting recombinant CK20 protein in a western blot assay. This antibody is similar to commercially available anti-Cytokeratin 20 antibody KS20.8 in staining various human tissues. This antibody was used in the immunohistochemical staining of stomach epithelial tissues and gastric adenocarcinomas (CN113583120).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for KRT20

Target: KRT20 (Keratin 20 (KRT20))

Alternative Name: Cytokeratin 20

Background: CK20, CK-20, K20, KRT20, Cytokeratin-20, Keratin-20, Keratin type I cytoskeletal 20 ,Protein IT, CGMCC NO 20764, KS20.8

UniProt: P35900