Recombinant HSP70 antibody (AA 450-463)

Catalog No. ABIN7543335

The Mouse Monoclonal anti-HSP70 antibody has been validated for WB, IHC, ELISA, IF and FACS. It is suitable to detect HSP70 in samples from Human.

Quick Overview for Recombinant HSP70 antibody (AA 450-463) (ABIN7543335)

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSP70 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: Cmhsp70-1

Product Details anti-HSP70 Antibody

Binding Specificity: AA 450-463

Purpose: Anti-HSP70 [cmhsp70.1 (C92F3B1)], Mouse IgG1, lambda

Specificity: This antibody binds a region including amino acids 473-504 (TKDNNLLGRFELSG) of the C-terminal substrate-binding domain (SBD) of membrane bound Hsp70 (mHsp70). It binds a 14-mer peptide termed"". This protein is located intracellularly and plays an important part in various cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. It plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. It maintains protein homeostasis during cellular stress through two opposing mechanisms: protein refolding and degradation. Membrane bound Hsp70s is frequently over-expressed in patients affected by leukemia and has been identified as a potential target for cancer therapies.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by grafting the CDRs of the mouse antibody on human framework regions. The parental mouse antibody was generated by immunizing mice with an SBD-derived synthetic peptide TKDNNLLGRFELSG (TKD peptide, aa 450-463) with complete Freud's adjuvants.

Isotype: IgG1 lambda

Application Details

Application Notes: The IgG1 version of this antibody binds the C-terminal substrate-binding domain (SBD) of the human membrane heat shock protein mHsp70 with an affinity of 3nM. The binding characterization of this antibody was done using ELISA. This antibody can detect aa 473-504) fused to domain III of HSA in a western blot assay (PMID: 20556545). The Fab version of this antibody binds mHsp70 with an affinity of 35 nM. The fab version can successfully identify membrane-associated Hsp70 on tumor cell lines both in immunofluorescence staining and flow cytometry (PMID: 20123884). The original mouse antibody cmHsp70.1 can selectively induce antibody-dependent cellular cytotoxicity (ADCC) of membrane Hsp70+ mouse tumor cells by unstimulated mouse spleen cells. Tumor killing could be further enhanced by activating the effector cells with TKD and IL-2 (PMID: 21187371). Cy5.5-conjugated cmHsp70.1 was capable of being endocytosed and accumulated in the tumor and was used for near-infrared fluorescence imaging of tumors. The tumor-selective internalization of mAb cmHsp70.1 might enable targeted uptake of toxins or radionuclides into Hsp70 membrane-positive tumors (PMID: 20406322). cmHsp70.1 antibody-conjugated gold nanoparticles were used in the real time imaging of small tumors (PMID: 26392771). This antibody was also used in the immunohistochemical and flow cytometric analysis of mHsp70 expressed on glioblastoma multiforme tumors (PMID: 29177668).

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for HSP70

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Alternative Name: HSP70

Background: HSP70-1, HSP70.1, HSPA1A, HSX70, HSP72, mHsp70, Heat shock 70 kDa protein 1A, Heat shock 70 kDa protein 1

UniProt: P0DMV8